Back to Search
Start Over
Isolation and functional characterization of proinflammatory acidic phospholipase A2 from Bothrops leucurus snake venom
- Source :
- Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. (3):226-233
- Publisher :
- Elsevier Inc.
-
Abstract
- In the present study, an acidic PLA(2), designated Bl-PLA(2), was isolated from Bothrops leucurus snake venom through two chromatographic steps: ion-exchange on CM-Sepharose and hydrophobic chromatography on Phenyl-Sepharose. Bl-PLA(2) was homogeneous on SDS-PAGE and when submitted to 2D electrophoresis the molecular mass was 15,000Da and pI was 5.4. Its N-terminal sequence revealed a high homology with other Asp49 acidic PLA(2)s from snake venoms. Its specific activity was 159.9U/mg and the indirect hemolytic activity was also higher than that of the crude venom. Bl-PLA(2) induced low myotoxic and edema activities as compared to those of the crude venom. Moreover, the enzyme was able to induce increments in IL-12p40, TNF-α, IL-1β and IL-6 levels and no variation of IL-8 and IL-10 in human PBMC stimulated in vitro, suggesting that Bl-PLA(2) induces proinflammatory cytokine production by human mononuclear cells. Bothrops leucurus venom is still not extensively explored and knowledge of its components will contribute for a better understanding of its action mechanism.
- Subjects :
- Male
Snake venom
Physiology
Health, Toxicology and Mutagenesis
Venom
Toxicology
Biochemistry
complex mixtures
Proinflammatory profile
Proinflammatory cytokine
Mice
Phospholipase A2
Crotalid Venoms
Animals
Edema
Humans
Bothrops
Amino Acid Sequence
Bothrops leucurus
Polyacrylamide gel electrophoresis
Cells, Cultured
Inflammation
biology
Molecular mass
Sequence Homology, Amino Acid
Interleukin-12 Subunit p40
Interleukin-6
Tumor Necrosis Factor-alpha
Sepharose
Interleukin-8
Cell Biology
General Medicine
biology.organism_classification
Chromatography, Ion Exchange
Interleukin-10
Molecular Weight
Phospholipases A2
biology.protein
Leukocytes, Mononuclear
Acidic phospholipase A2
Cytokines
Electrophoresis, Polyacrylamide Gel
lipids (amino acids, peptides, and proteins)
Subjects
Details
- Language :
- English
- ISSN :
- 15320456
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
- Accession number :
- edsair.doi.dedup.....d5092a2e8bff6a2a764ba6ba778c6b86
- Full Text :
- https://doi.org/10.1016/j.cbpc.2011.06.003