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Isolation and functional characterization of proinflammatory acidic phospholipase A2 from Bothrops leucurus snake venom

Authors :
Malson N. Lucena
Amélia Hamaguchi
Camila Takeno Cologna
Carlos Ueira-Vieira
Ana Carolina S. Oliveira
Maria Inês Homsi-Brandeburgo
Eliane Candiani Arantes
Veridiana M. Rodrigues
David N.S. Teixeira
Renata Santos Rodrigues
Débora Cristina de Oliveira Nunes
Source :
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. (3):226-233
Publisher :
Elsevier Inc.

Abstract

In the present study, an acidic PLA(2), designated Bl-PLA(2), was isolated from Bothrops leucurus snake venom through two chromatographic steps: ion-exchange on CM-Sepharose and hydrophobic chromatography on Phenyl-Sepharose. Bl-PLA(2) was homogeneous on SDS-PAGE and when submitted to 2D electrophoresis the molecular mass was 15,000Da and pI was 5.4. Its N-terminal sequence revealed a high homology with other Asp49 acidic PLA(2)s from snake venoms. Its specific activity was 159.9U/mg and the indirect hemolytic activity was also higher than that of the crude venom. Bl-PLA(2) induced low myotoxic and edema activities as compared to those of the crude venom. Moreover, the enzyme was able to induce increments in IL-12p40, TNF-α, IL-1β and IL-6 levels and no variation of IL-8 and IL-10 in human PBMC stimulated in vitro, suggesting that Bl-PLA(2) induces proinflammatory cytokine production by human mononuclear cells. Bothrops leucurus venom is still not extensively explored and knowledge of its components will contribute for a better understanding of its action mechanism.

Details

Language :
English
ISSN :
15320456
Issue :
3
Database :
OpenAIRE
Journal :
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Accession number :
edsair.doi.dedup.....d5092a2e8bff6a2a764ba6ba778c6b86
Full Text :
https://doi.org/10.1016/j.cbpc.2011.06.003