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Mechano-sensitivity of mitochondrial function in mouse cardiac myocytes
- Source :
- Progress in Biophysics and Molecular Biology. 130:315-322
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Mitochondria are an important source of reactive oxygen species (ROS). Although it has been reported that myocardial stretch increases cellular ROS production by activating nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2), referred to as X-ROS signalling, the involvement of mitochondria in X-ROS is not clear. Mitochondria are organelles that generate adenosine triphosphate (ATP) for cellular energy needs, which are mechanical-load-dependent. Therefore, it would not be surprising if these organelles had mechano-sensitive functions associated with stretch-induced ROS production. In the present study, we investigated the relation between X-ROS and mitochondrial stretch-sensitive responses in isolated mouse cardiac myocytes. The cells were subjected to 10% axial stretch using computer-controlled, piezo-manipulated carbon fibres attached to both cell ends. Cellular ROS production and mitochondrial membrane potential (Δψm) were assessed optically by confocal microscopy. The axial stretch increased ROS production and hyperpolarised Δψm. Treatment with a mitochondrial metabolic uncoupler, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP), at 0.5 μM did not suppress stretch-induced ROS production, whereas treatment with a respiratory Complex III inhibitor, antimycin A (5 μM), blunted the response. Although NOX inhibition by apocynin abrogated the stretch-induced ROS production, it did not suppress stretch-induced hyperpolarisation of Δψm. These results suggest that stretch causes activation of the respiratory chain to hyperpolarise Δψm, followed by NOX activation, which increases ROS production.
- Subjects :
- 0301 basic medicine
Biophysics
Respiratory chain
Antimycin A
Mitochondrion
Biology
Mice
03 medical and health sciences
chemistry.chemical_compound
Animals
Myocytes, Cardiac
Molecular Biology
Mechanical Phenomena
Membrane Potential, Mitochondrial
chemistry.chemical_classification
Reactive oxygen species
Biomechanical Phenomena
Mitochondria
Cell biology
030104 developmental biology
chemistry
Coenzyme Q – cytochrome c reductase
Apocynin
Reactive Oxygen Species
Adenosine triphosphate
Nicotinamide adenine dinucleotide phosphate
Subjects
Details
- ISSN :
- 00796107
- Volume :
- 130
- Database :
- OpenAIRE
- Journal :
- Progress in Biophysics and Molecular Biology
- Accession number :
- edsair.doi.dedup.....d51d804c2dcfbaa825500b7feaf61a48
- Full Text :
- https://doi.org/10.1016/j.pbiomolbio.2017.05.015