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Plasma Cell-Free Human Papillomavirus Oncogene E6 and E7 DNA Predicts Outcome in Oropharyngeal Squamous Cell Carcinoma
- Source :
- Journal of Molecular Diagnostics, 22(11), 1333-1343. Elsevier Science
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Persistent human papillomavirus (HPV) infection is associated with the development of oropharyngeal squamous cell carcinoma (OPSCC), and increasing incidences of OPSCC are reported. The generally favorable treatment outcome in patients with HPV-driven OPSCC has brought de-escalation of treatment into discussion. Nevertheless, 13% to 25% develop a relapse within two years after current standard treatment. New biomarkers are urgently required to monitor therapy response, tumor burden, and minimal residual disease during follow-up. This observational study examined 50 patients with OPSCC to investigate plasma cell-free (cf) HPV-DNA derived from tumor cells before therapy and during follow-up. Real-time quantitative PCR was applied to quantify the DNA concentration of HPV oncogenes E6 and E7. A total of 85.7% of pretreatment samples from patients with HPV-driven OPSCC (n = 28) were positive for at least one marker, and cfHPV-DNA concentration increased with tumor size. Virtually no signals were detected in HPV-negative OPSCC patients (n = 20; P ≤ 0.001). Patients without clinical evidence of recurrence had significantly reduced cfHPV-DNA concentrations after therapy (P ≤ 0.001). Conversely, cfHPV-DNA levels increased or remained above threshold in five patients who had residual disease or developed recurrence. In conclusion, plasma cfHPV-DNA detection correlates with the clinical course of disease in patients with HPV-driven OPSCC. Consequently, extensive clinical investigation should be considered if cfHPV-DNA is detected during follow-up of patients with HPV-driven OPSCC.
- Subjects :
- Male
0301 basic medicine
Oncology
Papillomavirus E7 Proteins
Pilot Projects
Disease
Alphapapillomavirus
Plasma cell
NSCLC
0302 clinical medicine
Medicine
NUCLEIC-ACIDS
RECURRENT
Oropharyngeal squamous cell carcinoma
Aged, 80 and over
Standard treatment
Middle Aged
Real-time polymerase chain reaction
medicine.anatomical_structure
Head and Neck Neoplasms
030220 oncology & carcinogenesis
SURVIVAL
Molecular Medicine
Female
Cell-Free Nucleic Acids
EXPRESSION
medicine.medical_specialty
Real-Time Polymerase Chain Reaction
Pathology and Forensic Medicine
03 medical and health sciences
P16(INK4A)
Internal medicine
Biomarkers, Tumor
Humans
BARR-VIRUS DNA
HEAD
Human papillomavirus
Aged
Oncogene
Squamous Cell Carcinoma of Head and Neck
business.industry
Papillomavirus Infections
HPV-DNA
P16
Oncogene Proteins, Viral
Minimal residual disease
Repressor Proteins
030104 developmental biology
DNA, Viral
Neoplasm Recurrence, Local
business
Follow-Up Studies
Subjects
Details
- ISSN :
- 15251578
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- The Journal of Molecular Diagnostics
- Accession number :
- edsair.doi.dedup.....d52e788a8113f98aa9d1190e20789b77
- Full Text :
- https://doi.org/10.1016/j.jmoldx.2020.08.002