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Novel Bioactive Peptides from Meretrix meretrix Protect Caenorhabditis elegans against Free Radical-Induced Oxidative Stress through the Stress Response Factor DAF-16/FOXO
- Source :
- Marine Drugs, Volume 16, Issue 11, Marine Drugs, Vol 16, Iss 11, p 444 (2018)
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- The hard clam Meretrix meretrix, which has been traditionally used as medicine and seafood, was used in this study to isolate antioxidant peptides. First, a peptide-rich extract was tested for its protective effect against paraquat-induced oxidative stress using the nematode model Caenorhabditis elegans. Then, three novel antioxidant peptides<br />MmP4 (LSDRLEETGGASS), MmP11 (KEGCREPETEKGHR) and MmP19 (IVTNWDDMEK), were identified and were found to increase the resistance of nematodes against paraquat. Circular dichroism spectroscopy revealed that MmP4 was predominantly in beta-sheet conformation, while MmP11 and MmP19 were primarily in random coil conformation. Using transgenic nematode models, the peptides were shown to promote nuclear translocation of the DAF-16/FOXO transcription factor, a pivotal regulator of stress response and lifespan, and induce the expression of superoxide dismutase 3 (SOD-3), an antioxidant enzyme. Analysis of DAF-16 target genes by real-time PCR reveals that sod-3 was up-regulated by MmP4, MmP11 and MmP19 while ctl-1 and ctl-2 were also up-regulated by MmP4. Further examination of daf-16 using RNA interference suggests that the peptide-increased resistance of C. elegans to oxidative stress was DAF-16 dependent. Taken together, these data demonstrate the antioxidant activity of M. meretrix peptides, which are associated with activation of the stress response factor DAF-16 and regulation of the antioxidant enzyme genes.
- Subjects :
- Paraquat
0301 basic medicine
Antioxidant
medicine.medical_treatment
Pharmaceutical Science
Caenorhabditis elegans
medicine.disease_cause
Meretrix meretrix
Article
Antioxidants
Animals, Genetically Modified
Biological Factors
03 medical and health sciences
chemistry.chemical_compound
RNA interference
antioxidant peptides
Drug Discovery
medicine
Animals
Caenorhabditis elegans Proteins
lcsh:QH301-705.5
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Transcription factor
reactive oxygen species
transcription factor DAF-16/FOXO
Cell Nucleus
chemistry.chemical_classification
Reactive oxygen species
biology
Forkhead Transcription Factors
biology.organism_classification
Bivalvia
Cell biology
Oxidative Stress
030104 developmental biology
Enzyme
lcsh:Biology (General)
Gene Expression Regulation
chemistry
Models, Animal
Biological Assay
RNA Interference
Peptides
Oxidative stress
Subjects
Details
- ISSN :
- 16603397
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Marine Drugs
- Accession number :
- edsair.doi.dedup.....d53179aec5ac53b5217c2f28562facfb
- Full Text :
- https://doi.org/10.3390/md16110444