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Boron Supplementation Promotes Osteogenesis of Tibia by Regulating the Bone Morphogenetic Protein-2 Expression in African Ostrich Chicks
- Source :
- Biological trace element research. 199(4)
- Publication Year :
- 2020
-
Abstract
- The present study aimed to explore the effects of supplemental boron on osteogenesis of tibia and to investigate the possible relationship between additional boron and the expression of bone morphogenetic protein-2 (BMP-2) in tibia of ostrich chicks. Therefore, forty-eight African ostrich chicks (15 days old) were supplemented with 0 mg/L, 40 mg/L, 80 mg/L, 160 mg/L, 320 mg/L, and 640 mg/L of boron in drinking water for 75 days. The paraffin sections of tibia used to measure histomorphometric parameters by hematoxylin and eosin (HE) staining, Masson’s staining, and immunohistochemistry (IHC). Enzyme-linked immunosorbent assay was performed to assess the level of BMP-2, osteocalcin (BGP), glucocorticoids (GCs), osteoprotegerin (OPG), and receptor activator of nuclear factor kappa-B ligand (RANKL) in serum. TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) technique was performed to detect the cell apoptosis. The results indicated that low dose of supplemental boron (40 mg/L–160 mg/L) in drinking water promotes bone development by increasing the mature ossein. The expression of BMP2 on 45 days was higher than 90 days. Serum level of BMP-2, BGP, and GCs changed significantly in groups with low dosage of boron, and OPG/RANKL ratio was upregulated from 0 to 160 mg/L. Cell apoptosis was least in 40 mg/L and 160 mg/L groups. Taken together, low dose of boron supplemented in drinking water could promote osteogenesis and growth and development of tibia by regulating the expression and secretion of BMP-2 and providing a dynamically balanced environment for tibia growth, development, and reconstruction by regulating the concentrations of BGP, GCs, and OPG/RANKL ratio in serum.
- Subjects :
- musculoskeletal diseases
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
Bone Morphogenetic Protein 2
010501 environmental sciences
01 natural sciences
Biochemistry
Bone morphogenetic protein 2
Inorganic Chemistry
Andrology
03 medical and health sciences
Osteoprotegerin
Osteogenesis
Animals
Tibia
Receptor
0105 earth and related environmental sciences
Boron
0303 health sciences
Struthioniformes
TUNEL assay
biology
Chemistry
030302 biochemistry & molecular biology
Biochemistry (medical)
RANK Ligand
General Medicine
RANKL
Dietary Supplements
Osteocalcin
biology.protein
Immunohistochemistry
Subjects
Details
- ISSN :
- 15590720
- Volume :
- 199
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biological trace element research
- Accession number :
- edsair.doi.dedup.....d53a72c0a1f086710e55eb3ad6925cc2