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Adjuvants recognized by toll-like receptors inhibit the induction of polarized type 2 T cell responses by natural attachment (G) protein of respiratory syncytial virus
- Source :
- Vaccine. 21:4348-4358
- Publication Year :
- 2003
- Publisher :
- Elsevier BV, 2003.
-
Abstract
- Immunization with native fusion (F) protein from respiratory syncytial virus (RSV) adsorbed to alum adjuvant generates greater than fourfold rises in serum neutralizing antibody titers in approximately 50% of seropositive humans. Using BALB/c mice we demonstrate herein that enhanced neutralization titers and accelerated clearance of virus from the lungs after challenge are possible if the attachment (G) glycoprotein is added to F protein-based vaccines. We further reveal for the first time that polarized type 2 T cell responses and immunopathology associated with G protein are inhibited by adjuvants recognized by toll-like receptors (TLR). Co-formulation with compounds that targeted TLR-2, TLR-3, TLR-4, or TLR-9 elicited significantly diminished type 2 T cell responses that caused granulocytic inflammation and eosinophilia in the airways after challenge. These results were not observed with recombinant IL-12 or QS-21. The data are important for improving combination vaccines for RSV.
- Subjects :
- Paramyxoviridae
T-Lymphocytes
medicine.medical_treatment
T cell
Receptors, Cell Surface
Viral Plaque Assay
medicine.disease_cause
Virus
Interferon-gamma
Mice
Adjuvants, Immunologic
Viral Envelope Proteins
medicine
Animals
Alum adjuvant
Neutralizing antibody
Mononegavirales
Lung
Mice, Inbred BALB C
Membrane Glycoproteins
General Veterinary
General Immunology and Microbiology
biology
Toll-Like Receptors
Public Health, Environmental and Occupational Health
Flow Cytometry
biology.organism_classification
Interleukin-12
Virology
Toll-Like Receptor 2
Toll-Like Receptor 3
Eosinophils
Toll-Like Receptor 4
Phenotype
Infectious Diseases
Respiratory syncytial virus (RSV)
medicine.anatomical_structure
Thrombopoietin
Respiratory Syncytial Virus, Human
biology.protein
Cytokines
Molecular Medicine
CpG Islands
Female
Adjuvant
Granulocytes
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....d53ecd6fab70091dc84b9b91c38c53b6