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Pharmacokinetics and bioavailability of gelsenicine in mice by UPLC-MS/MS
- Source :
- Biomedical chromatography : BMC. 33(3)
- Publication Year :
- 2018
-
Abstract
- Gelsenicine is an indole alkaloid isolated from Gelsemium elegans Benth. In recent years, the role of G. elegans Benth preparations in anti-tumor, analgesic, dilatation and dermatological treatment has attracted attention, and it has been applied clinically, but it is easy to cause poisoning with its use. An UPLC-MS/MS method was established to determine the gelsenicine in mouse blood, and the pharmacokinetics of gelsenicine after intravenous (0.1 mg/kg) and intragastric (0.5 and 1 mg/kg) administration was studied. Deltalin was used as internal standard; a UPLC BEH C18 column was used for chromatographic separation. The mobile phase consisted of acetonitrile and 10 mmol/L ammonium acetate (0.1% formic acid) with a gradient elution flow rate of 0.4 mL/min. Multiple reaction monitoring mode was used for quantitative analysis of gelsenicine in electrospray ionization positive interface. Proteins from mouse blood were removed by acetonitrile precipitation. A validation of this method was performed in accordance with the US Food and Drug Administration guidelines. In the concentration range of 0.05-100 ng/mL, the gelsenicine in the mouse blood was linear (r > 0.995), and the lower limit of quantification was 0.05 ng/mL. In the mouse blood, the intra-day precision RSD was 76.8%, and the matrix effect was between 103.7 and 108.4%, which meet the pharmacokinetic research requirements of gelsenicine. The UPLC-MS/MS method is sensitive, rapid and selective, and has been successfully applied to the pharmacokinetic study of gelsenicine in mice. The absolute bioavailability of gelsenicine is 1.13%.
- Subjects :
- Formic acid
Electrospray ionization
Clinical Biochemistry
Administration, Oral
Biological Availability
030226 pharmacology & pharmacy
01 natural sciences
Biochemistry
High-performance liquid chromatography
Analytical Chemistry
Indole Alkaloids
03 medical and health sciences
chemistry.chemical_compound
Mice
Random Allocation
0302 clinical medicine
Pharmacokinetics
Limit of Detection
Tandem Mass Spectrometry
Drug Discovery
Animals
Molecular Biology
Chromatography, High Pressure Liquid
Pharmacology
Mice, Inbred ICR
Chromatography
010401 analytical chemistry
Selected reaction monitoring
Reproducibility of Results
General Medicine
0104 chemical sciences
Bioavailability
chemistry
Linear Models
Administration, Intravenous
Quantitative analysis (chemistry)
Ammonium acetate
Subjects
Details
- ISSN :
- 10990801
- Volume :
- 33
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biomedical chromatography : BMC
- Accession number :
- edsair.doi.dedup.....d540224058b5cbc01f0c4b554f7914c5