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Ultrasensitive detection of long non-coding RNAs based on duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification
- Source :
- Chemical Communications. 55:6827-6830
- Publication Year :
- 2019
- Publisher :
- Royal Society of Chemistry (RSC), 2019.
-
Abstract
- We develop a new fluorescent method for sensitive detection of long noncoding RNAs (lncRNAs) on the basis of duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification. This method exhibits high sensitivity with a detection limit of 0.081 fM, and it can accurately detect the endogenous lncRNA HOTAIR in cancer cells, providing a new approach to study the physiological function of lncRNAs in human diseases.
- Subjects :
- 010402 general chemistry
Sensitivity and Specificity
01 natural sciences
Catalysis
Cell Line
Limit of Detection
Cell Line, Tumor
Materials Chemistry
Humans
Fluorescent Dyes
chemistry.chemical_classification
Detection limit
Nuclease
biology
010405 organic chemistry
Chemistry
Nucleic Acid Heteroduplexes
Metals and Alloys
RNA
HOTAIR
General Chemistry
Endonucleases
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Cell biology
Enzyme
Duplex (building)
Cancer cell
Ceramics and Composites
biology.protein
RNA, Long Noncoding
Nucleic Acid Amplification Techniques
Signal amplification
Subjects
Details
- ISSN :
- 1364548X and 13597345
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Chemical Communications
- Accession number :
- edsair.doi.dedup.....d58290fb6813ac23dfd55413fd38eb94