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A mouse model of renal ischemia-reperfusion injury solely induced by cold ischemia
- Source :
- Am J Physiol Renal Physiol
- Publication Year :
- 2019
-
Abstract
- Transplanted kidneys usually experience several episodes of ischemia, including cold ischemia during allograft storage in preservation solution. However, previous studies focusing on cold renal ischemia were only carried out in vitro or ex vivo. In the present study, we developed and characterized an in vivo mouse model of renal ischemia-reperfusion injury (IRI) induced exclusively by cold ischemia. C57BL/6 mice underwent right kidney nephrectomy, and the left kidney was kept cool with circulating cold saline in a kidney cup, while body temperature was maintained at 37°C. We clamped the renal pedicle and flushed out the blood inside the kidney with cold saline via an opening on the renal vein. The severity of renal IRI was examined with different ischemic durations. We found that the mice with
- Subjects :
- Male
medicine.medical_specialty
Time Factors
Physiology
Ischemia
urologic and male genital diseases
Kidney
Necrosis
Antigens, CD
Internal medicine
Medicine
Animals
cardiovascular diseases
Cold ischemia
Renal ischemia reperfusion
Kidney transplantation
business.industry
urogenital system
Cold Ischemia
Acute Kidney Injury
medicine.disease
Cadherins
Kidney Transplantation
Mice, Inbred C57BL
Disease Models, Animal
Creatinine
Reperfusion Injury
Cardiology
Disease Progression
business
Biomarkers
Glomerular Filtration Rate
Research Article
Subjects
Details
- ISSN :
- 15221466
- Volume :
- 317
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- American journal of physiology. Renal physiology
- Accession number :
- edsair.doi.dedup.....d586e1cd2926aa07bcfc548c5b289409