Production of Rabbit Anti-Rabies Immunoglobulin Using Cultural Antigen

Authors :: T. A. Mikheeva
L. N. Minaeva
I. M. Zhulidov
Zh. V. Matveeva
M. N. Kireev
L. V. Savitskaya
S. V. Generalov
A. K. Nikiforov
A. V. Komissarov
E. G. Abramova
M. V. Galkina
O. A. Lobovikova
Source :: Проблемы особо опасных инфекций, Vol 0, Iss 2(112), Pp 78-81 (2012)
Publication Year :: 2012
Publisher :: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”, 2012.
Abstract: Justified is the possibility of application of fixed rabies virus Moscow 3253, reproduced on the cell culture Vero, as an antigen for heterologous anti-rabies immunoglobulin production. Application of the adjuvant - aluminium hydrate - is determined to be effective. Herein, by the day 73 post immunization, specific antibodies titer is ≥ 1:500 (wherein specific activity has been identified by means of neutralization test, carried out on white mice, and dot-blot immunoassay). The level of specific activity in experimental samples of anti-rabies immunoglobulin, isolated from rabbit immune serum, corresponds to 332 and 347 ME/ml. Physicochemical and biological properties of anti-rabies immunoglobulin, produced with the help of cultural antigen, fully comply with regulatory requirements specified for commercial preparation of heterologous anti-rabies immunoglobulin.