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Characterization of lipids in complex samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry

Authors :
Josep M. Bayona
Udo A. Th. Brinkman
Eric Jover
Mohamed Adahchour
René J. J. Vreuls
BioAnalytical Chemistry
Source :
Journal of Chromatography A, 1086(1-2), 2-11. Elsevier, Jover, E, Adahchour, M, Bayona, J M, Vreuls, R J J & Brinkman, U A T 2005, ' Characterization of lipids in complex samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry ', Journal of Chromatography A, vol. 1086, no. 1-2, pp. 2-11 . https://doi.org/10.1016/j.chroma.2005.05.093
Publication Year :
2005

Abstract

Most lipids are a complex mixture of classes of compounds such as fatty acids, fatty alcohols, diols, sterols and hydroxy acids. In this study, the suitability of comprehensive two-dimensional gas chromatography coupled to a time-of-light mass spectrometer is studied for lipid characterization in complex samples. With lanolin, a refined wool wax, as test sample, it is demonstrated that combined methylation plus silylation is the preferred derivatization procedure to achieve (i) high-quality GC × GC separation and (ii) easily recognizable ordered structures in lipid analysis. Optimization of the GC × GC column combination, the influence of the temperature programme on the quality of the separation, and the potential and limitations of automated TOF-MS-based identification are discussed. The combined power of a 2D separation, ordered structures and MS detection is illustrated by the identification of several minor sample constituents. © 2005 Elsevier B.V. All rights reserved.

Details

Language :
English
ISSN :
00219673
Database :
OpenAIRE
Journal :
Journal of Chromatography A, 1086(1-2), 2-11. Elsevier, Jover, E, Adahchour, M, Bayona, J M, Vreuls, R J J & Brinkman, U A T 2005, ' Characterization of lipids in complex samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry ', Journal of Chromatography A, vol. 1086, no. 1-2, pp. 2-11 . https://doi.org/10.1016/j.chroma.2005.05.093
Accession number :
edsair.doi.dedup.....d5cf4c9082b10ea0fab204a7920e3d26
Full Text :
https://doi.org/10.1016/j.chroma.2005.05.093