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Inhibitory effects of crude extracts from some edible Thai plants against replication of hepatitis B virus and human liver cancer cells
- Source :
- BMC Complementary and Alternative Medicine, Vol 12, Iss 1, p 246 (2012), BMC Complementary and Alternative Medicine
- Publisher :
- Springer Nature
-
Abstract
- Background Edible plants such as Cratoxylum formosum (Jack) Dyer, Curcumin longa Lin, Momordica charantia Lin and Moringa oleifera Lam have long been believed in Thai culture to relieve ulcers and the symptoms of liver disease. However, little is known about their anti-liver cancer properties and antiviral activity against hepatitis B virus (HBV). The aim of this study was to investigate the anti-liver cancer and anti-HBV activities of crude extracts from these edible plants on human liver cancer cells. Methods Plant samples were prepared and extracted using buffer and hydro-alcoholic solvents. The MTT assay was performed to investigate the effects of the plant extracts on the cell viability of HepG2 cells. The inhibitory effect on replication of HBV was analysed by determining the level of HBV covalently closed circular DNA (cccDNA) in transiently transfected HepG2 cells with the DNA expression plasmid of the HBV genome using a quantitative real-time PCR. Results Buffer and hydroalcoholic extracts from C. formosum (leaf) reduced cell viability of HepG2 cells and they also inhibited HBV cccDNA. Crude extracts from C. longa (bulb) in both solvents did not have any cytotoxic effects on the HepG2 cells, but they significantly decreased the level of HBV cccDNA. Buffer extracts from the leaves of M. charantia and the fruits of M. oleifera showed to have anti-HBV activity and also a mild cytotoxicity effect on the HepG2 cells. In addition, leaves of M. Oleifera extracted by hydroalcoholic solvent drastically decreased the level of cccDNA in transiently transfected HepG2 cells. Conclusion Some crude extracts of edible plants contain compounds that demonstrate anti-liver cancer and anti-HBV activities.
- Subjects :
- Hepatitis B virus
Down-Regulation
medicine.disease_cause
Virus Replication
Antiviral Agents
Cell Line, Tumor
Anti-liver cancer
Medicine
Humans
MTT assay
Viability assay
Antiviral activity
Cytotoxicity
Momordica
biology
Traditional medicine
business.industry
Plant Extracts
HBV cccDNA
Liver Neoplasms
Cratoxylum formosum
Edible Thai plants
General Medicine
cccDNA
Hep G2 Cells
lcsh:Other systems of medicine
biology.organism_classification
Hepatitis B
Thailand
lcsh:RZ201-999
digestive system diseases
Complementary and alternative medicine
Cell culture
Plants, Edible
business
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14726882
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Complementary and Alternative Medicine
- Accession number :
- edsair.doi.dedup.....d5e79724a2f171a3109dcdd1d43c64e9
- Full Text :
- https://doi.org/10.1186/1472-6882-12-246