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Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation
- Source :
- Scientific Reports, SCIENTIFIC REPORTS(7), Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group UK, 2017.
-
Abstract
- T cells navigate diverse microenvironments to perform immune responses. Micro-scale topographical structures within the tissues, which may inherently exist in normal tissues or may be formed by inflammation or injury, can influence T cell migration, but how T cell migration is affected by such topographical structures have not been investigated. In this study, we fabricated ramp-like structures with a 5 μm height and various slopes, and observed T cells climbing up the ramp-like structures. T cells encountering the ramp-like structures exhibited MLC accumulation near head-tail junctions contacting the ramp-like structures, and made turns to the direction perpendicular to the ramp-like structures. Pharmacological study revealed that lamellipodia formation mediated by arp2/3 and contractility regulated by myosin light chain kinase (MLCK) were responsible for the intriguing turning behavior of T cells climbing the ramp-like structures. Arp2/3 or MLCK inhibition substantially reduced probability of T cells climbing sharp-edged ramp-like structures, indicating intriguing turning behavior of T cells mediated by lamellipodia formation and MLCK activity may be important for T cells to access inflamed or injured tissues with abrupt topographical changes.
- Subjects :
- 0301 basic medicine
Myosin light-chain kinase
T-Lymphocytes
lcsh:Medicine
Motility
macromolecular substances
Myosin light chain kinase activity
Article
Actin-Related Protein 2-3 Complex
03 medical and health sciences
Mice
0302 clinical medicine
Immune system
Cell Movement
Animals
Pseudopodia
lcsh:Science
Myosin-Light-Chain Kinase
Cells, Cultured
Multidisciplinary
Chemistry
musculoskeletal, neural, and ocular physiology
lcsh:R
Anatomy
030104 developmental biology
030220 oncology & carcinogenesis
Climbing
T cell migration
Biophysics
lcsh:Q
Lamellipodium
human activities
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....d5f4c8ec923498b743a9cd0ebe79d62c