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Beyond the 3′UTR binding–microRNA-induced protein truncation via DNA binding
- Source :
- Oncotarget, Oncotarget, 2018, Vol. 9, No. 67, pp: 32855-32867
- Publication Year :
- 2018
- Publisher :
- Impact Journals LLC, 2018.
-
Abstract
- Here, we present a miR mechanism which is active in the nucleus and is essential for the production of intron included, C-terminal truncated and biologically active proteins, like e.g. Vim3. We exemplified this mechanism by miRs, miR-15a and miR-498, which are overexpressed in clear cell renal carcinoma or oncocytoma. Both miRs directly interact with DNA in an intronic region, leading to transcriptional stop, and therefore repress the full length version of the pre-mRNA, resulting in intron included truncated proteins (Mxi-2 and Vim3). A computational survey shows that this miR:DNA interactions mechanism may be generally involved in regulating the human transcriptome, with putative interaction sites in intronic regions for over 1000 genes. In this work, an entirely new mechanism is revealed how miRs can repress full length protein translation, resulting in C-terminal truncated proteins.
- Subjects :
- 0301 basic medicine
miR-498
Transcriptome
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
ddc:570
microRNA
medicine
Mxi-2
Gene
miR-15
Chemistry
Three prime untranslated region
Intron
Biological activity
Cell biology
DNA interaction
030104 developmental biology
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Vim3
Nucleus
DNA
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 19492553
- Volume :
- 9
- Issue :
- 67
- Database :
- OpenAIRE
- Journal :
- Oncotarget
- Accession number :
- edsair.doi.dedup.....d623c89006d9501b219ac2c9c580bf56