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Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG)
- Publication Year :
- 2011
- Publisher :
- Elsevier Science Limited, 2011.
-
Abstract
- To evaluate the presence of Toxoplasma gondii in edible farmed shellfish, 1734 shellfish specimens i.e., 109 Crassostrea gigas (6 pools), 660 Mytilus galloprovincialis (22 pools), 804 Tapes decussatus (28 pools) and 161 Tapes philippinarum (6 pools), were collected from the Varano Lagoon (Apulia, Italy). Shellfish from 62 pools were subjected to two molecular techniques: a nested-PCR assay, and a fluorescent amplicon generation (FLAG) real-time PCR assay, both based on the multi-copy B1 target, were performed. One pooled sample of gills from C. gigas and one pooled sample of haemolymphs from T. decussatus were assessed as positive for T. gondii DNA by both techniques. The results demonstrated the presence of T. gondii in edible farmed C. gigas and T. decussatus and indicate that there may be a considerable health threat involved in eating contaminated raw shellfish.
- Subjects :
- Veterinary medicine
animal structures
Immunology
Toxoplama gondii, Edible shellfish, Nested-PCR, FLAG real-time PCR, Italy
FLAG real-time PCR
Aquaculture
Polymerase Chain Reaction
Food Parasitology
Toxoplama gondii
Edible shellfish
Nested-PCR
Animals
Crassostrea
Shellfish
Mytilus
Base Sequence
biology
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
business.industry
food and beverages
Toxoplasma gondii
DNA
General Medicine
Toxoplama gondii Edible shellfish Nested-PCR FLAG real-time PCR Italy
DNA, Protozoan
Amplicon
biology.organism_classification
Bivalvia
Fishery
Infectious Diseases
Real-time polymerase chain reaction
Italy
Mollusca
Parasitology
business
Toxoplasma
Nested polymerase chain reaction
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....d627a6edb9e68b1d8961639f6fdc24c3