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Chasing away accurate results: exhaustive chase protocols underestimate maximum metabolic rate estimates in European perch <scp> Perca fluviatilis </scp>
- Source :
- Journal of Fish Biology
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Metabolic rates are one of many measures that are used to explain species' response to environmental change. Static respirometry is used to calculate the standard metabolic rate (SMR) of fish, and when combined with exhaustive chase protocols it can be used to measure maximum metabolic rate (MMR) and aerobic scope (AS) as well. While these methods have been tested in comparison to swim tunnels and chambers with circular currents, they have not been tested in comparison with a no‐chase control. We used a repeated‐measures design to compare estimates of SMR, MMR and AS in European perch Perca fluviatilis following three protocols: (a) a no‐chase control; (b) a 3‐min exhaustive chase; and (c) a 3‐min exhaustive chase followed by 1‐min air exposure. We found that, contrary to expectations, exhaustive chase protocols underestimate MMR and AS at 18°C, compared to the no‐chase control. This suggests that metabolic rates of other species with similar locomotorty modes or lifestyles could be similarly underestimated using chase protocols. These underestimates have implications for studies examining metabolic performance and responses to climate change scenarios. To prevent underestimates, future experiments measuring metabolic rates should include a pilot with a no‐chase control or, when appropriate, an adjusted methodology in which trials end with the exhaustive chase instead of beginning with it.
- Subjects :
- 0106 biological sciences
Biosensing Techniques
Aquatic Science
Biology
Zoologi
010603 evolutionary biology
01 natural sciences
methods
Respirometry
Oxygen Consumption
Statistics
Regular Paper
Animals
aerobic scope
Biological sciences
Swimming
Ecology, Evolution, Behavior and Systematics
Ekologi
Perch
Ecology
exhaustive chase
010604 marine biology & hydrobiology
Regular Papers
standard metabolic rate
biology.organism_classification
climate change
Air exposure
Perches
intermittent-flow respirometry
Metabolic rate
Fish
intermittent‐flow respirometry
Zoology
Subjects
Details
- ISSN :
- 10958649 and 00221112
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- Journal of Fish Biology
- Accession number :
- edsair.doi.dedup.....d62f731f0bdc1af40ee6485d11fefb71