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Determination of bisphenol A in canned food by microwave assisted extraction, molecularly imprinted polymer-solid phase extraction and liquid chromatography-mass spectrometry
- Source :
- Journal of Chromatography B. 1137:121938
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Bisphenol A (BPA), a known potential endocrine disrupting compound (EDC) is expected to be present in low quantities in canned food due to its migration from the inner surface coating of cans made of epoxy resins. A selective and confirmatory analytical method, based on microwave assisted extraction (MAE), molecularly imprinted solid phase extraction (MISPE) using a polymer prepared by a non-covalent molecular imprinting technique and liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI/MS) was developed for the determination of BPA in canned pineapple, tuna and mushrooms. First, the effect of the loading medium of hydro- organic solutions on the binding of BPA and its deuterated analogue on the MISPE sorbent was investigated. Subsequently, the effects of the experimental conditions of the microwave assisted extraction (solvent, sample mass/solvent volume, time and temperature) on the obtained recovery of BPA from canned food were assessed and the parameters were optimized to provide maximum recovery and selectivity. It was demonstrated that the combination of MAE with MISPE permits the use of a selective extraction solvent (methanol/water, 4/6, v/v), simplifying the sample preparation steps and enhancing sample clean-up of complex food matrices. The method was validated in different food matrices, using BPA-d16 as internal standard and quantitative relative recoveries were determined. The precision (RSD %) of the method ranged from 7% to 10% and the limit of detection was at low ng/g level for all food matrices. The determined concentration of BPA in commercial canned samples ranged between 7.3 and 42.3 ng/g.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Clinical Biochemistry
030226 pharmacology & pharmacy
01 natural sciences
Biochemistry
High-performance liquid chromatography
Analytical Chemistry
Molecular Imprinting
03 medical and health sciences
0302 clinical medicine
Phenols
Endocrine disrupting compound
Limit of Detection
Liquid chromatography–mass spectrometry
Food, Preserved
Sample preparation
Solid phase extraction
Benzhydryl Compounds
Microwaves
Chromatography, High Pressure Liquid
Chromatography
Chemistry
Solid Phase Extraction
010401 analytical chemistry
Extraction (chemistry)
Molecularly imprinted polymer
Reproducibility of Results
Cell Biology
General Medicine
0104 chemical sciences
Surface coating
Linear Models
Subjects
Details
- ISSN :
- 15700232
- Volume :
- 1137
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography B
- Accession number :
- edsair.doi.dedup.....d673f77737f9614410403b3438603af9