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Range of cell-wall alterations enhance saccharification in [i]Brachypodium distachyon[/i] mutants
- Source :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2014, 111 (40), pp.14601-14606. ⟨10.1073/pnas.1414020111⟩, Proceedings of the National Academy of Sciences of the United States of America, 2014, 111 (40), pp.14601-14606. ⟨10.1073/pnas.1414020111⟩, www.pnas.org, Proceedings of the National Academy of Sciences of the United States of America 40 (111), 14601-14606. (2014)
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- Lignocellulosic plant biomass is an attractive feedstock for the production of sustainable biofuels, but the commercialization of such products is hampered by the high costs of processing this material into fermentable sugars (saccharification). One approach to lowering these costs is to produce crops with cell walls that are more susceptible to hydrolysis to reduce preprocessing and enzyme inputs. To deepen our understanding of the molecular genetic basis of lignocellulose recalcitrance, we have screened a mutagenized population of the model grass Brachypodium distachyon for improved saccharification with an industrial polysaccharide-degrading enzyme mixture. From an initial screen of 2,400 M2 plants, we selected 12 lines that showed heritable improvements in saccharification, mostly with no significant reduction in plant size or stem strength. Characterization of these putative mutants revealed a variety of alterations in cell-wall components. We have mapped the underlying genetic lesions responsible for increased saccharification using a deep sequencing approach, and here we report the mapping of one of the causal mutations to a narrow region in chromosome 2. The most likely candidate gene in this region encodes a GT61 glycosyltransferase, which has been implicated in arabinoxylan substitution. Our work shows that forward genetic screening provides a powerful route to identify factors that impact on lignocellulose digestibility, with implications for improving feedstock for cellulosic biofuel production.
- Subjects :
- [SDV]Life Sciences [q-bio]
Mutant
Population
Biomass
lignin
Computational biology
7. Clean energy
Chromosomes, Plant
chemistry.chemical_compound
Cell Wall
Polysaccharides
Arabinoxylan
Spectroscopy, Fourier Transform Infrared
lignocellulosic biofuel
matrix polysaccharides
feruloylation
education
Cellulose
Plant Proteins
2. Zero hunger
education.field_of_study
Principal Component Analysis
Multidisciplinary
biology
Plant Stems
business.industry
Monosaccharides
Chromosome Mapping
Glycosyltransferases
food and beverages
Biological Sciences
biology.organism_classification
lignine
Biotechnology
saccharification
chemistry
Cellulosic ethanol
Biofuel
biocarburant
Biofuels
Mutation
Carbohydrate Metabolism
Brachypodium
Brachypodium distachyon
business
Subjects
Details
- Language :
- English
- ISSN :
- 00278424 and 10916490
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2014, 111 (40), pp.14601-14606. ⟨10.1073/pnas.1414020111⟩, Proceedings of the National Academy of Sciences of the United States of America, 2014, 111 (40), pp.14601-14606. ⟨10.1073/pnas.1414020111⟩, www.pnas.org, Proceedings of the National Academy of Sciences of the United States of America 40 (111), 14601-14606. (2014)
- Accession number :
- edsair.doi.dedup.....d677e6217e99c601b81de885cc277bf6