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Actomyosin dynamics, Bmp and Notch signaling pathways drive apical extrusion of proepicardial cells
- Publication Year :
- 2018
- Publisher :
- Cold Spring Harbor Laboratory, 2018.
-
Abstract
- The epicardium, the outer mesothelial layer enclosing the myocardium, plays key roles in heart development and regeneration. During embryogenesis it arises from the proepicardium (PE), a cell cluster that appears in the dorsal pericardium close to the venous pole of the heart. Little is known about how the PE emerges from the pericardial mesothelium. Using the zebrafish model and a combination of genetic tools, pharmacological agents and quantitativein vivoimaging we reveal that a coordinated collective movement of the dorsal pericardium drives PE formation. We found that PE cells are apically extruded in response to actomyosin activity. Our results reveal that the coordinated action of Notch/Bmp pathways is critically needed for apical extrusion of PE cells. More generally, by comparison to cell extrusion for the elimination of unfit cells from epithelia, our results describe a unique mechanism where extruded cell viability is maintained.
- Subjects :
- 0303 health sciences
Heart development
biology
Chemistry
Regeneration (biology)
Embryogenesis
Cell
Notch signaling pathway
biology.organism_classification
Cell biology
03 medical and health sciences
0302 clinical medicine
medicine.anatomical_structure
medicine
Pericardium
Viability assay
Zebrafish
030217 neurology & neurosurgery
030304 developmental biology
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....d6794f534383b537f970769b557d7284