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Label-free optical sensor based on red blood cells laser tweezers Raman spectroscopy analysis for ABO blood typing
- Source :
- Optics express. 24(21)
- Publication Year :
- 2016
-
Abstract
- The clinical significance of ABO blood typing extends beyond transfusion medicine and is demonstrated to be associated with susceptibility to various diseases, even including cancer. In this study, a home-made laser tweezers Raman spectroscopy (LTRS) system was applied to detect red blood cells (RBCs) with the aim to develop a label-free, simple and objective blood typing method for the first time. High-quality Raman spectra of RBCs in the fingerprint region of 420-1700 cmsup-1/supcan be obtained, meanwhile exciting blood typing results can be achieved, especially with an accuracy of 100% for identifying Type AB from other blood types with the use of multivariate statistical analysis based on principal component analysis (PCA) combined with linear discriminant analysis (LDA). This primary work demonstrates that the label-free RBCs LTRS analysis in conjunction with PCA-LDA diagnostic algorithms has great potential as a biosensor for ABO blood typing.
- Subjects :
- medicine.medical_specialty
Materials science
Erythrocytes
02 engineering and technology
Spectrum Analysis, Raman
01 natural sciences
Blood typing
symbols.namesake
Optics
hemic and lymphatic diseases
ABO blood group system
Neoplasms
medicine
Humans
Label free
Principal Component Analysis
business.industry
010401 analytical chemistry
Discriminant Analysis
Diagnostic algorithms
Transfusion medicine
Surface-enhanced Raman spectroscopy
021001 nanoscience & nanotechnology
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Optical tweezers
Blood Grouping and Crossmatching
symbols
Erythrocyte Count
0210 nano-technology
business
Raman spectroscopy
Algorithms
Biomedical engineering
Subjects
Details
- ISSN :
- 10944087
- Volume :
- 24
- Issue :
- 21
- Database :
- OpenAIRE
- Journal :
- Optics express
- Accession number :
- edsair.doi.dedup.....d736be1b77cca91af6a295fa7321d9ec