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Deferiprone attenuates inflammation and myocardial fibrosis in diabetic cardiomyopathy rats
- Source :
- Biochemical and biophysical research communications. 486(4)
- Publication Year :
- 2017
-
Abstract
- We attempted to investigate the therapeutic effects of deferiprone on DC rats and explore the underlying mechanism. Total 24 6-week-old male Wistar rats (weighing from 180 g to 220 g) were subjected to DC model construction and then randomly divided to three groups (8 rats per group): DC group, DC + 50 mg, and DC + 100 mg deferiprone treatment group. The 8 normal rats were considered as controls. After deferiprone treatment for 20 weeks, the blood samples were collected for the biochemical parameters test, including fasting glucose, HOMA-IR (homeostasis model assessment of the insulin resistance), serum iron, ferritin and transferrin saturation (TS). The oxidative stress was assessed by detecting the level of malondialdehyde (MDA) and superoxide dismutase (SOD). Histopathologic changes were determined by Masson's trichrome staining and electron microscopy imaging. The expression levels of NF-κB (nuclear factor kappa B), COX2 (cytochrome c oxidase), tenascin C, collagen IV were measured by RT-PCR and western blotting. The expression of nitrotyrosine and MCP-1 (monocyte chemotactic protein 1) were determined by immunohistochemistry. Deferiprone treatment reduced iron deposition and IR in DC rats except for blood glucose. After deferiprone treatment, MDA level was significantly decreased and SOD level was increased significantly. The level of NF-κB, cyclooxygenase-2, tenascin C, collagen IV MCP-1 and nitrotyrosine were significantly reduced. There was no significant difference in the effect of deferiprone at 50 and 100 mg doses. Deferiprone showed therapeutic effects on DC by regulating the pro-inflammatory and pro-fibrotic factors.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Diabetic Cardiomyopathies
Pyridones
Biophysics
030204 cardiovascular system & hematology
Iron Chelating Agents
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Insulin resistance
Internal medicine
Diabetic cardiomyopathy
medicine
Animals
Immunologic Factors
Deferiprone
Rats, Wistar
Molecular Biology
biology
medicine.diagnostic_test
Dose-Response Relationship, Drug
Transferrin saturation
business.industry
Nitrotyrosine
Cell Biology
medicine.disease
Malondialdehyde
Endomyocardial Fibrosis
Rats
Ferritin
Myocarditis
Oxidative Stress
030104 developmental biology
Endocrinology
Treatment Outcome
chemistry
Immunology
biology.protein
Serum iron
business
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 486
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....d7b167821c8df2cf58133563ab5c6e8b