Back to Search
Start Over
Global miRNA expression is temporally correlated with acute kidney injury in mice
- Source :
- PeerJ, Vol 4, p e1729 (2016), PeerJ
- Publication Year :
- 2016
- Publisher :
- PeerJ Inc., 2016.
-
Abstract
- MicroRNAs (miRNAs) are negative regulators of gene expression and protein abundance. Current evidence shows an association of miRNAs with acute kidney injury (AKI) leading to substantially increased morbidity and mortality. Here, we investigated whether miRNAs are inductive regulators responsible for the pathological development of AKI. Microarray analysis was used to detect temporal changes in global miRNA expression within 48 h after AKI in mice. Results indicated that global miRNA expression gradually increased over 24 h from ischemia reperfusion injury after 24 h, and then decreased from 24 h to 48 h. A similar trend was observed for the index of tubulointerstitial injury and the level of serum creatinine, and there was a significant correlation between the level of total miRNA expression and the level of serum creatinine (p< 0.05). This expression-phenotype correlation was validated by quantitative reverse transcription PCR on individual miRNAs, including miR-18a, -134, -182, -210 and -214. Increased global miRNA expression may lead to widespread translational repression and reduced cellular activity. Furthermore, significant inflammatory cytokine release and peritubular capillary loss were observed, suggesting that the initiation of systematic destruction programs was due to AKI. Our findings provide new understanding of the dominant role of miRNAs in promoting the pathological development of AKI.
- Subjects :
- 0301 basic medicine
Microarray
medicine.medical_treatment
Temporal expression
lcsh:Medicine
Biology
Bioinformatics
General Biochemistry, Genetics and Molecular Biology
Andrology
03 medical and health sciences
chemistry.chemical_compound
microRNA
Gene expression
medicine
Pathology
Creatinine
Microarray analysis techniques
General Neuroscience
lcsh:R
Acute kidney injury
MicroRNA
General Medicine
Cell Biology
medicine.disease
Serum creatinine
030104 developmental biology
Cytokine
chemistry
Nephrology
General Agricultural and Biological Sciences
Reperfusion injury
Subjects
Details
- Language :
- English
- ISSN :
- 21678359
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- PeerJ
- Accession number :
- edsair.doi.dedup.....d7b976d68e4a6b93ac4d98ff2e2974af