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Enhanced cellular internalization and gene silencing with a series of cationic dendron-multiwalled carbon nanotube:siRNA complexes
- Source :
- The FASEB Journal; Vol 24, FASEB Journal, FASEB Journal, Federation of American Society of Experimental Biology, 2010, 24 (11), pp.4354-65. ⟨10.1096/fj.09-141036⟩
- Publication Year :
- 2010
- Publisher :
- FEDERATION AMER SOC EXP BIOL, 2010.
-
Abstract
- International audience; One of the major obstacles to the clinical development of gene silencing by small interfering RNA (siRNA) is its effective cytoplasmic delivery. Carbon nanotubes have been proposed as novel nanomaterials that can offer significant advantages for the intracellular delivery of nucleic acids, such as siRNA. We recently demonstrated in a proof-of-principle study that amino-functionalized multiwalled carbon nanotubes (f-MWNT) can effectively deliver in vivo an siRNA sequence, triggering cell apoptosis that results in human lung xenograft eradication and prolonged survival. In the present study, we demonstrate how a newly synthesized series of polycationic dendron-MWNT constructs with a precisely tailored number of amino functions (dendron generations) can complex and effectively deliver double-stranded siRNA to achieve gene silencing in vitro. A systematic comparison between the f-MWNT series in terms of cellular uptake, cytotoxicity, and siRNA complexation is offered. Significant improvement in siRNA delivery with the dendron-MWNT conjugates is shown, and gene silencing was obtained in 2 human cell lines using 2 different siRNA sequences. The study reveals that through f-MWNT structure-biological function analysis novel nanotube-based siRNA transfer vectors can be designed with minimal cytotoxicity and effective delivery and gene-silencing capabilities.
- Subjects :
- Models, Molecular
Small interfering RNA
MESH: Cell Line, Tumor
Cell Survival
MESH: Biological Transport
02 engineering and technology
Biology
Transfection
010402 general chemistry
01 natural sciences
Biochemistry
Cell Line
[SDV.SP.MED]Life Sciences [q-bio]/Pharmaceutical sciences/Medication
RNA interference
Cations
Cell Line, Tumor
MESH: RNA, Small Interfering
Genetics
Humans
Gene silencing
MESH: Gene Silencing
Gene Silencing
RNA, Small Interfering
MESH: Cations
Cytotoxicity
Molecular Biology
Gene knockdown
MESH: Humans
carbon nanotubes
Nanotubes, Carbon
MESH: Transfection
RNA
Biological Transport
021001 nanoscience & nanotechnology
MESH: Cell Line
0104 chemical sciences
Cell biology
MESH: Hela Cells
MESH: Cell Survival
MESH: Nanotubes, Carbon
Drug delivery
0210 nano-technology
MESH: Models, Molecular
HeLa Cells
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 15306860 and 08926638
- Volume :
- 24
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- The FASEB Journal
- Accession number :
- edsair.doi.dedup.....d7cddaafc9463ae41c195c17395f9cc0
- Full Text :
- https://doi.org/10.1096/fj.09-141036