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Effect of engineered nanoparticles on vasomotor responses in rat intrapulmonary artery
- Source :
- Toxicology and Applied Pharmacology, Toxicology and Applied Pharmacology, Elsevier, 2010, 245 (2), pp.203-210. ⟨10.1016/j.taap.2010.03.002⟩, Toxicology and Applied Pharmacology, 2010, 245 (2), pp.203-10. ⟨10.1016/j.taap.2010.03.002⟩, Toxicology and Applied Pharmacology, Elsevier, 2010, 245 (2), pp.203-10. ⟨10.1016/j.taap.2010.03.002⟩
- Publication Year :
- 2010
- Publisher :
- HAL CCSD, 2010.
-
Abstract
- International audience; Pulmonary circulation could be one of the primary vascular targets of finest particles that can deeply penetrate into the lungs after inhalation. We investigated the effects of engineered nanoparticles on vasomotor responses of small intrapulmonary arteries using isometric tension measurements. Acute in vitro exposure to carbon nanoparticles (CNP) decreased, and in some case abolished, the vasomotor responses induced by several vasoactive agents, whereas acute exposure to titanium dioxide nanoparticles (TiO(2)NP) did not. This could be attributed to a decrease in the activity of those vasoactive agents (including PGF(2)(alpha), serotonin, endothelin-1 and acetylcholine), as suggested when they were exposed to CNP before being applied to arteries. Also, CNP decreased the contraction induced by 30 mM KCl, without decreasing its activity. After endoplasmic reticulum calcium stores depletion (by caffeine and thapsigargin), CaCl(2) addition induced a contraction, dependent on Store-Operated Calcium Channels that was not modified by acute CNP exposure. Further addition of 30 mM KCl elicited a contraction, originating from activation of Voltage-Operated Calcium Channels that was diminished by CNP. Contractile responses to PGF(2)(alpha) or KCl, and relaxation to acetylcholine were modified neither in pulmonary arteries exposed in vitro for prolonged time to CNP or TiO(2)NP, nor in those removed from rats intratracheally instilled with CNP or TiO(2)NP. In conclusion, prolonged in vitro or in vivo exposure to CNP or TiO(2)NP does not affect vasomotor responses of pulmonary arteries. However, acute exposure to CNP decreases contraction mediated by activation of Voltage-Operated, but not Store-Operated, Calcium Channels. Moreover, interaction of some vasoactive agents with CNP decreases their biological activity that might lead to misinterpretation of experimental data.
- Subjects :
- Male
Contraction (grammar)
Vasodilator Agents
[SDV]Life Sciences [q-bio]
02 engineering and technology
MESH: Rats, Sprague-Dawley
Toxicology
Endoplasmic Reticulum
[SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
MESH: Dose-Response Relationship, Drug
Rats, Sprague-Dawley
chemistry.chemical_compound
Vasoconstrictor Agents
MESH: Animals
MESH: Isometric Contraction
ComputingMilieux_MISCELLANEOUS
Titanium
0303 health sciences
Inhalation Exposure
Vasomotor
Voltage-dependent calcium channel
Biological activity
021001 nanoscience & nanotechnology
MESH: Titanium
MESH: Calcium Channels
MESH: Inhalation Exposure
0210 nano-technology
Acetylcholine
medicine.drug
medicine.medical_specialty
Thapsigargin
MESH: Rats
MESH: Pulmonary Artery
chemistry.chemical_element
MESH: Carbon
Calcium
Pulmonary Artery
03 medical and health sciences
MESH: Vasoconstrictor Agents
In vivo
MESH: Endoplasmic Reticulum
Internal medicine
Isometric Contraction
medicine
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Rats, Wistar
030304 developmental biology
Pharmacology
Dose-Response Relationship, Drug
MESH: Rats, Wistar
Carbon
MESH: Male
Rats
MESH: Vasodilator Agents
Endocrinology
chemistry
Nanoparticles
Calcium Channels
MESH: Nanoparticles
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0041008X and 10960333
- Database :
- OpenAIRE
- Journal :
- Toxicology and Applied Pharmacology, Toxicology and Applied Pharmacology, Elsevier, 2010, 245 (2), pp.203-210. ⟨10.1016/j.taap.2010.03.002⟩, Toxicology and Applied Pharmacology, 2010, 245 (2), pp.203-10. ⟨10.1016/j.taap.2010.03.002⟩, Toxicology and Applied Pharmacology, Elsevier, 2010, 245 (2), pp.203-10. ⟨10.1016/j.taap.2010.03.002⟩
- Accession number :
- edsair.doi.dedup.....d7fce369ab619ac2e2181a3b3797c592