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Salt responsive alternative splicing of a RING finger E3 ligase modulates the salt stress tolerance by fine-tuning the balance of COP9 signalosome subunit 5A
- Source :
- PLoS Genetics, PLoS Genetics, Vol 17, Iss 11, p e1009898 (2021)
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- Increasing evidence points to the tight relationship between alternative splicing (AS) and the salt stress response in plants. However, the mechanisms linking these two phenomena remain unclear. In this study, we have found that Salt-Responsive Alternatively Spliced gene 1 (SRAS1), encoding a RING-Type E3 ligase, generates two splicing variants: SRAS1.1 and SRAS1.2, which exhibit opposing responses to salt stress. The salt stress-responsive AS event resulted in greater accumulation of SRAS1.1 and a lower level of SRAS1.2. Comprehensive phenotype analysis showed that overexpression of SRAS1.1 made the plants more tolerant to salt stress, whereas overexpression of SRAS1.2 made them more sensitive. In addition, we successfully identified the COP9 signalosome 5A (CSN5A) as the target of SRAS1. CSN5A is an essential player in the regulation of plant development and stress. The full-length SRAS1.1 promoted degradation of CSN5A by the 26S proteasome. By contrast, SRAS1.2 protected CSN5A by competing with SRAS1.1 on the same binding site. Thus, the salt stress-triggered AS controls the ratio of SRAS1.1/SRAS1.2 and switches on and off the degradation of CSN5A to balance the plant development and salt tolerance. Together, these results provide insights that salt-responsive AS acts as post-transcriptional regulation in mediating the function of E3 ligase.<br />Author summary High salinity severely affects plant growth and development, impairing crop production worldwide. E3 ligase is a stress-responsive regulator through ubiquitin-proteasome system for selective protein degradation. The E3s are regulated by transcriptional regulation and post-translational modifications. Here, we have discovered that stress-responsive AS acts as a post-transcriptional regulation modulating the function of E3 ligases. Intriguingly, the truncated proteins generated by salt-responsive AS play opposite roles compared with the full-length E3 ligase. The truncated isoform losing key domain could not degrade the target protein, instead, it interacts and competes with the E3 ligase through binding the same domain of the targets. This finding contributes significantly to a deeper mechanistic understanding of how AS regulates the function of E3 ligase in response to salt stress.
- Subjects :
- Salinity
Leaves
Cancer Research
Physiology
Arabidopsis
Plant Science
QH426-470
Plant Reproduction
Salt Stress
Biochemistry
Genetically Modified Plants
Ligases
Cell Signaling
Plant Resistance to Abiotic Stress
Seed Germination
Gene expression
Protein Isoforms
Arabidopsis thaliana
Genetics (clinical)
Ecology
biology
Plant Anatomy
Genetically Modified Organisms
Eukaryota
Plants
Signaling Cascades
Enzymes
Cell biology
Ubiquitin ligase
medicine.anatomical_structure
Experimental Organism Systems
Plant Physiology
RNA splicing
Engineering and Technology
Genetic Engineering
Research Article
Signal Transduction
Biotechnology
Ubiquitin-Protein Ligases
Arabidopsis Thaliana
Bioengineering
Brassica
Genes, Plant
Research and Analysis Methods
Stress Signaling Cascade
Model Organisms
Plant and Algal Models
Plant-Environment Interactions
Genetics
Ring finger
medicine
Plant Defenses
COP9 signalosome
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Arabidopsis Proteins
COP9 Signalosome Complex
Plant Ecology
Ecology and Environmental Sciences
Alternative splicing
Organisms
Biology and Life Sciences
Proteins
Cell Biology
Plant Pathology
biology.organism_classification
Alternative Splicing
Proteasome
Seedlings
Enzymology
Animal Studies
biology.protein
Plant Biotechnology
Subjects
Details
- ISSN :
- 15537404
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- PLOS Genetics
- Accession number :
- edsair.doi.dedup.....d80377496643ce3c5c7dc89d4dabc57a