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DNA:RNA hybrid G-quadruplex formation upstream of transcription start site
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-6 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- Bioinformatic analysis reveals an enrichment of putative DNA:RNA hybrid G-quadruplex-forming sequences (PHQS) on both sides of the transcription start sites (TSSs) in the genome of warm-blooded animals, suggesting a positive selection of PHQSs in evolution and functional role of DNA:RNA hybrid G-quadruplexes (HQs) in transcription. The formation of HQs downstream of TSS in transcribed DNA has been documented under in vitro conditions; however, it is still not known if such HQs can form at the upstream side of TSSs. In this study, we report that such HQs can form in transcription in DNA with two to three guanine tracts if RNA carrying the required number of G-tracts is supplied. We also show that the formation of such HQs is dependent on the negative supercoiling generated by RNA polymerases. These results suggest that HQs may also form at the upstream side of TSSs in vivo and play a role in transcription since the two requirements are satisfied in cells.
- Subjects :
- 0301 basic medicine
Guanine
Transcription, Genetic
Oligonucleotides
lcsh:Medicine
010402 general chemistry
G-quadruplex
01 natural sciences
Genome
Article
03 medical and health sciences
chemistry.chemical_compound
Transcription (biology)
Humans
lcsh:Science
Polymerase
Multidisciplinary
Base Sequence
biology
lcsh:R
Computational Biology
RNA
DNA
DNA-Directed RNA Polymerases
0104 chemical sciences
Cell biology
G-Quadruplexes
030104 developmental biology
chemistry
biology.protein
Nucleic Acid Conformation
DNA supercoil
lcsh:Q
Transcription Initiation Site
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....d80ae42732de9c85a42d73add8b222d1