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Pru p 3-Glycodendropeptides Based on Mannoses Promote Changes in the Immunological Properties of Dendritic and T-Cells from LTP-Allergic Patients
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2019
- Publisher :
- Wiley-Blackwell, 2019.
-
Abstract
- SCOPE Glycodendropeptides (GDPs) functionalized with mannose can enhance allergen interaction with dendritic cells (DCs) via C-type lectin receptors (CLRs), modulating the immune response. They can present multiple peptides and have potential applications for diagnosis and treatment of food allergy (FA). The immune response induced by GDPs with mannose and Pru p 3 peptides (mono/tetravalent) with ester (D1 ManPrup3/D4 ManPrup3) or ether linkers (D1 Man-O- Prup3/D4 Man-O- Prup3) in lipid-transfer-protein-allergic patients and tolerant controls is analyzed. METHODS AND RESULTS The immunological response induced by GDPs is studied by assessing monocyte-derived-DC maturation, lymphocyte proliferation, cytokine production, and basophil response by flow cytometry. Dn ManPrup3 was recognized by DCs via CLRs inducing DC maturation in all subjects. However, CCR7 expression is significantly upregulated in allergic patients compared to tolerant controls. These changes correlate with lymphocyte proliferation and specific production of Th2/Th1 cytokines in allergic patients. Moreover, D1 ManPrup3 does not induce basophil activation. CONCLUSION Dn ManPrup3 induces changes in DC maturation and lymphocyte proliferation, indicating specific recognition via CLRs. Prup3-GDPs are recognized by immune cells, inducing a specific immune response and modulating the immunological response in FA patients. The specific geometry of D1 ManPrup3 in particular makes it a potential candidate for specific immunotherapy development.
- Subjects :
- 0301 basic medicine
lymphocytes
Adult
Male
medicine.medical_treatment
T-Lymphocytes
C-C chemokine receptor type 7
Lymphocyte proliferation
Basophil
food allergies
Lymphocyte Activation
03 medical and health sciences
Immune system
medicine
Humans
Lectins, C-Type
Receptor
Plant Proteins
030109 nutrition & dietetics
Chemistry
mannose
Glycopeptides
Dendritic Cells
Antigens, Plant
Middle Aged
Acquired immune system
Basophils
Basophil activation
030104 developmental biology
Cytokine
medicine.anatomical_structure
Immunology
Cytokines
Female
C-type lectin receptors
Carrier Proteins
Mannose
Food Hypersensitivity
Food Science
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Accession number :
- edsair.doi.dedup.....d84e239fb36b7cadff6662620141b550