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A method for lactate and pyruvate determination in filter-paper dried blood spots
- Source :
- Journal of chromatography. A. 1216(51)
- Publication Year :
- 2009
-
Abstract
- Lactic acidemia is commonly associated with severe diseases in pediatric patients. Quantitation of blood lactate and pyruvate is important for the diagnosis and clinical management. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using dried blood spots (DBS) was developed and could be used for simultaneous quantification of blood lactate and pyruvate. The applicability of the developed method was tested and confirmed by the regression analysis between LC-MS/MS method and enzymatic assay. Lactate and pyruvate were extracted from DBS obtained from 580 full-term, 120 pre-term infants (gestations ranging from 24 to 36 weeks), and 65 patients with suspected lactic acidemia, with methanolic internal standard (IS) solutions of sodium L-lactate-(13)C(3) and pyruvate-(13)C(3). An API-2000 LC-MS/MS system with multiple reaction monitoring (MRM) mode was applied. The within-run and between-run precisions (CV%) were determined and the results were 1.9% and 3.9% for lactate (n=20) and 5.7% and 7.3% for pyruvate (n=20). The linearity of lactate (r=0.9986) and pyruvate (r=0.9973) based on the IS was excellent. The parameter r squared (r(2)) of linear regression between LC-MS/MS method and enzymatic assay was 0.9405 for lactate and 0.9447 for pyruvate, respectively, and the agreement between these methods was consistent and acceptable. The stability of lactate and pyruvate on DBS was also confirmed. The LC-MS/MS method we developed is a specific, sensitive, and reproducible method for measuring blood lactate and pyruvate concentrations. The use of DBS in this method makes it particularly attractive for pediatric patients.
- Subjects :
- Paper
Sodium
chemistry.chemical_element
Biochemistry
Analytical Chemistry
Liquid chromatography–mass spectrometry
Reference Values
Tandem Mass Spectrometry
Pyruvic Acid
Humans
Lactic Acid
Dried blood
Enzyme Assays
Chromatography
biology
Spots
Filter paper
Chemistry
Organic Chemistry
Selected reaction monitoring
Infant, Newborn
General Medicine
Reference Standards
Enzyme assay
Dried blood spot
Calibration
biology.protein
Regression Analysis
Filtration
Chromatography, Liquid
Subjects
Details
- ISSN :
- 18733778
- Volume :
- 1216
- Issue :
- 51
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography. A
- Accession number :
- edsair.doi.dedup.....d852b11783715fcbc55c0c647edb34a2