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Coiled-Coil Peptide Beacon
- Source :
- Mueller, C & Grossmann, T N 2018, ' Coiled-Coil Peptide Beacon : A Tunable Conformational Switch for Protein Detection ', Angewandte Chemie. International Edition, vol. 57, no. 52, pp. 17079-17083 . https://doi.org/10.1002/anie.201811515, Angewandte Chemie International Edition, Angewandte Chemie (International Ed. in English), Angewandte Chemie. International Edition, 57(52), 17079-17083. Wiley
- Publication Year :
- 2018
-
Abstract
- The understanding of protein folding and assembly is of central importance for the design of proteins and enzymes with novel or improved functions. Minimalistic model systems, such as coiled‐coils, provide an excellent platform to improve this understanding and to construct novel molecular devices. Along those lines, we designed a conformational switch that is composed of two coiled‐coil forming peptides and a central binding epitope. In the absence of a binding partner, this switch adopts a hairpin‐like conformation that opens upon receptor binding. Variation of the coiled‐coil length modulates the strength of the intramolecular constraint. The two conformational states of this switch have been linked with characteristic fluorescent properties, which enables the detection of the receptor in real‐time.
- Subjects :
- Models, Molecular
Protein Folding
Protein Conformation
conformational constraint
Peptide
molecular beacons
protein–protein interactions
010402 general chemistry
01 natural sciences
Fluorescence
Catalysis
Epitope
Protein–protein interaction
molecular devices
Molecular beacon
heterodimeric coiled-coil
Coiled coil
chemistry.chemical_classification
Chemistry
010405 organic chemistry
Communication
Proteins
General Chemistry
General Medicine
Communications
0104 chemical sciences
Molecular Devices | Hot Paper
Intramolecular force
Biophysics
Protein folding
Peptides
Subjects
Details
- Language :
- English
- ISSN :
- 14337851
- Volume :
- 57
- Issue :
- 52
- Database :
- OpenAIRE
- Journal :
- Angewandte Chemie. International Edition
- Accession number :
- edsair.doi.dedup.....d8bfd4b4cb1f37726f934d2d0734c6f2
- Full Text :
- https://doi.org/10.1002/anie.201811515