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Inonotus obliquus polysaccharide ameliorates impaired reproductive function caused by Toxoplasma gondii infection in male mice via regulating Nrf2-PI3K/AKT pathway
- Source :
- International journal of biological macromolecules. 151
- Publication Year :
- 2019
-
Abstract
- This study was carried out to investigate the effects of Inonotus obliquus polysaccharide (IOP) on impaired reproductive function and its mechanisms in Toxoplasma gondii (T. gondii)-infected male mice. Results showed that IOP significantly improved the spermatogenic capacity and ameliorated pathological damage of testis, increased serum testosterone (T), luteinizing hormone (LH) and follicular-stimulating hormone (FSH) levels in T. gondii-infected male mice. IOP effectively up-regulated testicular steroidogenic acute regulatory protein (StAR), P450scc and 17β-HSD expressions. IOP also significantly decreased the levels of malondialdehyde (MDA) and nitric oxide (NO), but increased the activities of antioxidant enzyme superoxide dismutase (SOD) and glutathione (GSH). Furthermore, IOP up-regulated the expressions of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1) and NADPH quinoneoxidoreductase-1 (NQO-1), and suppressed the apoptosis of testicular cells by decreasing Bcl-2 associated x protein (Bax) and cleaved caspase-3 expressions. IOP further enhanced testicular phosphatidylinositol 3-kinase (PI3K), phospho-protein kinase B (p-AKT) and phospho-mammalian target of rapamycin (p-mTOR) expression levels. It demonstrates the beneficial effects of IOP on impaired reproductive function in T. gondii-infected male mice due to its anti-oxidative stress and anti-apoptosis via regulating Nrf2-PI3K/AKT signaling pathway.
- Subjects :
- Male
endocrine system
medicine.medical_specialty
NF-E2-Related Factor 2
Apoptosis
02 engineering and technology
Chemical Fractionation
Biochemistry
Nitric oxide
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Mice
Phosphatidylinositol 3-Kinases
Structural Biology
Internal medicine
Testis
medicine
Animals
Gonadal Steroid Hormones
Molecular Biology
PI3K/AKT/mTOR pathway
030304 developmental biology
0303 health sciences
biology
Sperm Count
Akt/PKB signaling pathway
Steroidogenic acute regulatory protein
Reproduction
Fungal Polysaccharides
General Medicine
Glutathione
021001 nanoscience & nanotechnology
Spermatozoa
Inonotus
Oxidative Stress
Endocrinology
chemistry
biology.protein
0210 nano-technology
Luteinizing hormone
Proto-Oncogene Proteins c-akt
Toxoplasma
Biomarkers
Toxoplasmosis
Signal Transduction
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 151
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....d8ca9cd6a6bf00b2b16dea4df332774c