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Functional analysis target of rapamycin (TOR) on the Penaeus vannamei in response to acute low temperature stress
- Source :
- Fishshellfish immunology. 96
- Publication Year :
- 2019
-
Abstract
- Target of rapamycin (TOR) is an atypical of Ser/Thr protein kinase that plays an important role in many aspects such as cell growth, reproduction, differentiation, cell cycle regulation, autophagy and apoptosis. However, little information is known about the enzyme in crustaceans. Here, a novel TOR was identified from shrimp Penaeus vannamei (PvTOR) and its biological functions were investigated in response low temperature stress. The PvTOR gene encoded a polypeptide of 2464 amino acids with an estimated molecular mass of 279.4 kD and a predicted isoelectronic point (pI) of 7.30. Phylogenetic analysis revealed that PvTOR shared high similarity with other known species. PvTOR mRNA was detected in all the tested tissues and highest transcription in muscle and hepatopancreas. PvTOR transcriptional level was up-regulated significantly at 1.5 h and 3 h, and down-regulated at 12 h and 24 h after low temperature stress. TEM and autophagy indicator system GFP-PvLC3 suggested that low temperature induced autophagy generation. ROS, Ca2+ concentration and apoptosis rate were increased significantly in TOR-knockdown shrimp after low temperature stress. The autophagy associated gene ATG8II/I, PvBeclin-1, PvATG14, apoptosis gene PvPARP, Pvcasp-3, PvBAX and Pvp53 transcripts, and casp-3/8 activity in hemocyte were increased significantly in TOR-knockdown group shrimp at 3 h after low temperature stress. Additionally, THC counts of TOR-knockdown group were significantly higher than the dsGFP group. In summary, these results suggested that PvTOR plays an important role in the adaptation mechanisms of shrimp at low temperature by regulating autophagy and apoptosis.
- Subjects :
- 0301 basic medicine
Apoptosis
Aquatic Science
Biology
Arthropod Proteins
03 medical and health sciences
Penaeidae
Transcription (biology)
Stress, Physiological
Autophagy
Environmental Chemistry
Animals
Protein kinase A
Gene
Phylogeny
Cell growth
TOR Serine-Threonine Kinases
04 agricultural and veterinary sciences
General Medicine
Sequence Analysis, DNA
Cell cycle
Cell biology
Shrimp
Cold Temperature
030104 developmental biology
040102 fisheries
0401 agriculture, forestry, and fisheries
Signal Transduction
Subjects
Details
- ISSN :
- 10959947
- Volume :
- 96
- Database :
- OpenAIRE
- Journal :
- Fishshellfish immunology
- Accession number :
- edsair.doi.dedup.....d8cd54d91d532239829c67348a7934a5