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Essential oil-loaded lipid nanoparticles for wound healing
- Source :
- International Journal of Nanomedicine
- Publication Year :
- 2017
- Publisher :
- Informa UK Limited, 2017.
-
Abstract
- Francesca Saporito,1 Giuseppina Sandri,1 Maria Cristina Bonferoni,1 Silvia Rossi,1 Cinzia Boselli,1 Antonia Icaro Cornaglia,2 Barbara Mannucci,3 Pietro Grisoli,1 Barbara Vigani,1 Franca Ferrari1 1Department of Drug Sciences, University of Pavia, Pavia, 2Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, 3Centro Grandi Strumenti, University of Pavia, Pavia, Italy Abstract: Chronic wounds and severe burns are diseases responsible for severe morbidity and even death. Wound repair is a crucial process and tissue regeneration enhancement and infection prevention are key factors to minimize pain, discomfort, and scar formation. The aim of this work was the development of lipid nanoparticles (solid lipid nanoparticles and nanostructured lipid carriers [NLC]), to be loaded with eucalyptus or rosemary essential oils and to be used, as medical devices, to enhance healing of skin wounds. Lipid nanoparticles were based on natural lipids: cocoa butter, as solid lipid, and olive oil or sesame oil, as liquid lipids. Lecithin was chosen as surfactant to stabilize nanoparticles and to prevent their aggregation. The systems were prepared by high shear homogenization followed by ultrasound application. Nanoparticles were characterized for physical–chemical properties, bioadhesion, cytocompatibility, in vitro proliferation enhancement, and wound healing properties toward normal human dermal fibroblasts. Antimicrobial activity of nanoparticles was evaluated against two reference microbial strains, one of Staphylococcus aureus, the other of Streptococcus pyogenes. Finally, the capability of nanoparticles to promote wound healing in vivo was evaluated on a rat burn model. NLC based on olive oil and loaded with eucalyptus oil showed appropriate physical–chemical properties, good bioadhesion, cytocompatibility, in vitro proliferation enhancement, and wound healing properties toward fibroblasts, associated to antimicrobial properties. Moreover, the in vivo results evidenced the capability of these NLC to enhance the healing process. Olive oil, which is characterized by a high content of oleic acid, proved to exert a synergic effect with eucalyptus oil with respect to antimicrobial activity and wound repair promotion. Keywords: lipid nanoparticles, eucalyptus essential oil, antimicrobial properties, wound healing
- Subjects :
- Male
antimicrobial properties
eucalyptus essential oil
Pharmaceutical Science
02 engineering and technology
lipid nanoparticles
01 natural sciences
Lecithin
law.invention
chemistry.chemical_compound
Anti-Infective Agents
International Journal of Nanomedicine
law
Lecithins
Drug Discovery
Food science
Original Research
Drug Carriers
Eucalyptus
Chemistry
General Medicine
021001 nanoscience & nanotechnology
Antimicrobial
Lipids
Eucalyptus oil
0210 nano-technology
Staphylococcus aureus
food.ingredient
Streptococcus pyogenes
Biophysics
Bioengineering
Biomaterials
food
In vivo
Solid lipid nanoparticle
Oils, Volatile
Animals
Humans
Rats, Wistar
Essential oil
Cell Proliferation
Wound Healing
Organic Chemistry
Fibroblasts
Rats
0104 chemical sciences
010404 medicinal & biomolecular chemistry
Oleic acid
Nanoparticles
Wound healing
Subjects
Details
- ISSN :
- 11782013
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- International Journal of Nanomedicine
- Accession number :
- edsair.doi.dedup.....d8dab15a86623ae998dd229cc448e4ca