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p53 Dependent Apoptotic Cell Death Induces Embryonic Malformation in Carassius auratus under Chronic Hypoxia
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 7, p e102650 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- Hypoxia is a global phenomenon affecting recruitment as well as the embryonic development of aquatic fauna. The present study depicts hypoxia induced disruption of the intrinsic pathway of programmed cell death (PCD), leading to embryonic malformation in the goldfish, Carrasius auratus. Constant hypoxia induced the early expression of pro-apoptotic/tumor suppressor p53 and concomitant expression of the cell death molecule, caspase-3, leading to high level of DNA damage and cell death in hypoxic embryos, as compared to normoxic ones. As a result, the former showed delayed 4 and 64 celled stages and a delay in appearance of epiboly stage. Expression of p53 efficiently switched off expression of the anti-apoptotic Bcl-2 during the initial 12 hours post fertilization (hpf) and caused embryonic cell death. However, after 12 hours, simultaneous downregulation of p53 and Caspase-3 and exponential increase of Bcl-2, caused uncontrolled cell proliferation and prevented essential programmed cell death (PCD), ultimately resulting in significant (p
- Subjects :
- Embryology
Programmed cell death
lcsh:Medicine
Epiboly
Apoptosis
Aquaculture
Biology
Bioinformatics
Cell Fate Determination
Congenital Abnormalities
Downregulation and upregulation
Goldfish
medicine
Animals
lcsh:Science
Hypoxia
Cell Proliferation
Multidisciplinary
Caspase 3
Cell growth
lcsh:R
Embryogenesis
Biology and Life Sciences
Agriculture
Cell Differentiation
Hypoxia (medical)
Embryonic stem cell
Cell biology
Proto-Oncogene Proteins c-bcl-2
Somites
Chronic Disease
lcsh:Q
Tumor Suppressor Protein p53
medicine.symptom
Research Article
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....d8fc00e415b8866fa5ba83ffcb15ebc4