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Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand
- Source :
- Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021), Scientific Reports
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Malaria remains a public health problem in Thailand, especially along its borders where highly mobile populations can contribute to persistent transmission. This study aimed to determine resistant genotypes and phenotypes of 112 Plasmodium falciparum isolates from patients along the Thai-Cambodia border during 2013–2015. The majority of parasites harbored a pfmdr1-Y184F mutation. A single pfmdr1 copy number had CVIET haplotype of amino acids 72–76 of pfcrt and no pfcytb mutations. All isolates had a single pfk13 point mutation (R539T, R539I, or C580Y), and increased % survival in the ring-stage survival assay (except for R539I). Multiple copies of pfpm2 and pfcrt-F145I were detected in 2014 (12.8%) and increased to 30.4% in 2015. Parasites containing either multiple pfpm2 copies with and without pfcrt-F145I or a single pfpm2 copy with pfcrt-F145I exhibited elevated IC90 values of piperaquine. Collectively, the emergence of these resistance patterns in Thailand near Cambodia border mirrored the reports of dihydroartemisinin-piperaquine treatment failures in the adjacent province of Cambodia, Oddar Meanchey, suggesting a migration of parasites across the border. As malaria elimination efforts ramp up in Southeast Asia, host nations militaries and other groups in border regions need to coordinate the proposed interventions.
- Subjects :
- Male
0301 basic medicine
Endemic Diseases
Drug Resistance
Protozoan Proteins
Parasitemia
medicine.disease_cause
law.invention
0302 clinical medicine
Genotype-phenotype distinction
law
Malaria, Falciparum
Genetics
Mutation
Multidisciplinary
Middle Aged
Thailand
Artemisinins
Transmission (mechanics)
Quinolines
Medicine
Drug Therapy, Combination
Female
Adult
Adolescent
DNA Copy Number Variations
Genotype
Science
Plasmodium falciparum
030106 microbiology
030231 tropical medicine
Biology
Article
Antimalarials
Young Adult
03 medical and health sciences
Piperaquine
parasitic diseases
medicine
Humans
Parasite genetics
Genetic Association Studies
Aged
Point mutation
Haplotype
DNA, Protozoan
medicine.disease
biology.organism_classification
Malaria
Haplotypes
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....d905fe1033a232c527ac88606efb831b