The effect of cold stress on polycystic ovary syndrome in rat: before and during modeling

The aim of this study was the evaluation effects of a chronic intermittent cold stress regimen before and during PCO modeling in rat. Forty-eight rats were treated with estradiol valerate for 60 days in order to induction of follicular cysts (PCO modeling). Rats were divided into two groups: (1) before (cold stress 3 weeks then PCO modeling) and (2) during modeling (cold stress and PCO simultaneous). The biochemical and histological findings show that in simultaneous cold stress and estradiol injection group PCOS modeling has been done successfully and in the other group cold stress prevents induction of PCO in rats. The present study is the first survey that reports 3-week chronic stress before estradiol injection prevents the induction of PCO in rats.