In vitro Cultivation of Porcine Kidney

Summary and Conclusions1. Some of the nutritional requirements for the in vitro cultivation of hog kidney epithelial cells were determined. The data indicate that cultures grown in a “simple” medium containing Synthetic Mixture #199 (92.5%) and hog serum (7.5%) were consistently more proliferative than cultures cultivated in more complex media containing embryo extract, serum ultra-filtrate and serum of homologous and heterologous origin. 2. None of the media tested adequately supported growth of either splenic or testicular fibroblasts. 3. The influence of various Porcine Serum Fractions (II, IV, V and IV + V) on growth of hog kidney epithelial cells was also investigated. When Fraction IV -f- V was used, excellent growth was achieved. When Fraction II, IV or V was used independently, growth inferior to that of whole serum resulted.