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Polysaccharides from Pyracantha fortuneana and its biological activity
- Source :
- International Journal of Biological Macromolecules. 150:1162-1174
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- This study used response surface methodology to determine the optimal conditions for extraction of polysaccharides from Pyracantha. fortuneana (PSPF), and studied the mechanism of PSPF-inducing apoptosis in human ovarian carcinoma Skov3 cells. Response surface methodology (RSM) were adopted to extract PSPF. The maximum value of polysaccharide yield was obtained under these optimal conditions. PSPF had good potential as an antioxidant. Exposure of cells to PSPF resulted in cytotoxicity through the induction of apoptosis, and the reactive oxygen species were increased, mitochondrial membrane potential decreased, DNA damage (detected as γ- H2AX and RAD51 foci) was observed in Skov3 cells. In addition, PSPF could induce apoptosis of cancer cells. Therefore, PSPF should be explored as novel potential antioxidants and an anti-tumor drug in a clinical setting.
- Subjects :
- Antioxidant
DNA damage
medicine.medical_treatment
Apoptosis
Pyracantha fortuneana
02 engineering and technology
Polysaccharide
Biochemistry
Antioxidants
03 medical and health sciences
Polysaccharides
Structural Biology
Cell Line, Tumor
medicine
Humans
Cytotoxicity
Molecular Biology
030304 developmental biology
Ovarian Neoplasms
chemistry.chemical_classification
0303 health sciences
Reactive oxygen species
biology
General Medicine
021001 nanoscience & nanotechnology
biology.organism_classification
Antineoplastic Agents, Phytogenic
Neoplasm Proteins
chemistry
Cancer cell
Female
Pyracantha
Reactive Oxygen Species
0210 nano-technology
DNA Damage
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 150
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....d96dd6be2177a963627d4c6b46f4361d