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Difference mid-IR nanospectroscopy on individual patches of purple membranes: the proton pump activity of bacteriorhodopsin at the nanoscale

Authors :
Peter Hegemann
Michele Ortolani
Matthias Broser
Ulrich Schade
Leonetta Baldassarre
Laura Zanetti-Polzi
Eglof Ritter
Ljiljana Puskar
Paolo Biagioni
Valeria Giliberti
Stefano Corni
Francesco Rusconi
Isabella Daidone
Raffaella Polito
Source :
44th International Conference on Infrared, Millimeter, and Terahertz Waves, IRMMW-THz 2019, Maison de la Chimie Paris; France, 1-6/09/2019, info:cnr-pdr/source/autori:Giliberti V.; Polito R.; Ritter E.; Broser M.; Hegemann P.; Puskar L.; Schade U.; Zanetti-Polzi L.; Daidone I.; Corni S.; Rusconi F.; Biagioni P.; Baldassarre L.; Ortolani M./congresso_nome:44th International Conference on Infrared, Millimeter, and Terahertz Waves, IRMMW-THz 2019/congresso_luogo:Maison de la Chimie Paris; France/congresso_data:1-6%2F09%2F2019/anno:2019/pagina_da:/pagina_a:/intervallo_pagine
Publication Year :
2019
Publisher :
IEEE, 2019.

Abstract

Conformational changes of the light-sensitive membrane receptor and proton pump bacteriorhodopsin is investigated by tip-enhanced mid-infrared nanospectroscopy on individual cell membrane patches, with a dramatic increase of sensitivity (in terms of number of probed molecules) compared with standard mid-infrared spectroscopy.

Details

Database :
OpenAIRE
Journal :
2019 44th International Conference on Infrared, Millimeter, and Terahertz Waves (IRMMW-THz)
Accession number :
edsair.doi.dedup.....d9ae645bccb8e7d46b6a6c9eab8e8f07