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Natural killer cell deficiency in patients with non-Hodgkin lymphoma after lung transplantation
- Source :
- The Journal of Heart and Lung Transplantation. 34:604-612
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Background Post-transplant non-Hodgkin lymphoma (NHL) is a well-recognized complication of solid-organ transplantation, and pharmacologic suppression of adaptive immunity plays a major role in its development. However, the role of natural killer (NK) cells in post-lung transplant de novo NHL is unknown. Methods Extensive phenotypic analyses of NK cells from patients diagnosed with NHL after liver or lung transplantation were conducted with multicolor flow cytometry. Polyfunctionality assays simultaneously assessed NK cell degranulation (CD107a) and intracellular cytokine production (interferon-γ and tumor necrosis factor-α) in the presence of NHL target cells. Results The development of de novo NHL is linked to NK-cell maturation defects, including overexpression of NKG2A and CD62L and down-modulation of inhibitory killer immunoglobulin-like receptors and CD57 receptors. More importantly, in patients who developed NHL after lung transplantation, we observed a specific down-modulation of the activating receptors (NKp30, NKp46, and NKG2D) and a sharp decrease in perforin expression and degranulation against NHL target cells. Conclusions Our results suggest that accumulation of abnormal NK cells could play a role in the outgrowth of NHL after lung transplantation, independently of the immunosuppressive regimen.
- Subjects :
- Male
Pulmonary and Respiratory Medicine
Natural killer cell
Interleukin 21
Postoperative Complications
immune system diseases
hemic and lymphatic diseases
medicine
Humans
Transplantation
biology
business.industry
Lymphoma, Non-Hodgkin
Degranulation
Middle Aged
medicine.disease
NKG2D
Acquired immune system
Lymphoma
Killer Cells, Natural
medicine.anatomical_structure
Perforin
Immunology
biology.protein
Female
Surgery
Cardiology and Cardiovascular Medicine
business
Lung Transplantation
Subjects
Details
- ISSN :
- 10532498
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- The Journal of Heart and Lung Transplantation
- Accession number :
- edsair.doi.dedup.....d9bd4db4ab481613b5df0223932dc4d4