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Rapid identification of bacterial species with bacterial DNA microarray in cirrhotic patients with spontaneous bacterial peritonitis
- Source :
- Internal medicine (Tokyo, Japan). 48(1)
- Publication Year :
- 2009
-
Abstract
- Background/Aims Early detection and identification of bacteria in ascitic fluid could result in more timely treatment of cirrhotic patients with spontaneous bacterial peritonitis (SBP) or subclinical SBP. The aim of this study was to evaluate the usefulness of a bacterial DNA microarray for the rapid diagnosis of SBP and rapid bacterial identification in cirrhotic patients with ascites. Methods Thirty-seven cirrhotic patients with ascites (25 men and 12 women) participated. Ascitic fluid obtained from patients was tested by the bacterial DNA microarray method and by the conventional culture method. Results SBP and bacterascites were diagnosed in 8 (16.7%) of 48 specimens by the conventional method. The bacterial DNA microarray proved the existence of bacteria in 6 (75%) of 8 samples with SBP or bacterascites using the conventional method as a gold standard. A corresponding rate of bacterial species identification between the two methods was found in 5 of 6 samples (83.3%). It took 1.47±0.96 and 5.14±2.6 days to receive the data by the microarray and conventional method, respectively (p
- Subjects :
- Adult
DNA, Bacterial
Liver Cirrhosis
Male
Time Factors
Microarray
Peritonitis
Microbiology
Spontaneous bacterial peritonitis
Ascites
Internal Medicine
medicine
Ascitic Fluid
Humans
Subclinical infection
Aged
Oligonucleotide Array Sequence Analysis
Aged, 80 and over
biology
business.industry
General Medicine
Gold standard (test)
Bacterial Infections
Middle Aged
medicine.disease
biology.organism_classification
Female
medicine.symptom
DNA microarray
business
Bacteria
Subjects
Details
- ISSN :
- 13497235
- Volume :
- 48
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Internal medicine (Tokyo, Japan)
- Accession number :
- edsair.doi.dedup.....d9be5301c921e7cb740d6f654cc38f3c