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Evaluation of protective effect of recombinant dense granule antigens GRA2 and GRA6 formulated in monophosphoryl lipid A (MPL) adjuvant against Toxoplasma chronic infection in mice
- Source :
- Vaccine, Vaccine, Elsevier, 2007, 25 (21), pp.4301-11. ⟨10.1016/j.vaccine.2007.02.057⟩
- Publication Year :
- 2007
- Publisher :
- HAL CCSD, 2007.
-
Abstract
- To investigate the vaccine potential of both the Toxoplasma GRA2 and GRA6 antigens, the full length recombinant proteins were produced in Escherichia coli, formulated in MPL adjuvant, and used alone and in combination ("mix"), to immunize CBA/J mice. Although high ratios of specific IgG2a/IgG1 were measured against both proteins, only spleen cells from GRA2-immunized mice and mix-immunized mice produced high amounts of both IFN-gamma and IL-2 upon induction with Toxoplasma gondii Excretory-Secretory Antigens. Intra peritoneal challenge with Toxoplasma cysts resulted in significant reduction of brain cysts in GRA2- and in mix-vaccinated mice only. This study shows the protective efficacy of recombinant GRA2 against chronic infection by T. gondii and confirms the utility of MPL adjuvant in enabling a vaccine candidate to induce a protective Th1 immune response.
- Subjects :
- Protozoan Vaccines
medicine.medical_treatment
Protozoan Proteins
Antibodies, Protozoan
Monophosphoryl Lipid A
MESH: Vaccines, Subunit
MESH: Adjuvants, Immunologic
MESH: Lipid A
law.invention
Mice
0302 clinical medicine
law
MESH: Animals
MESH: Protozoan Proteins
Vaccines, Synthetic
0303 health sciences
MESH: Cytokines
MESH: Mice, Inbred CBA
MESH: Toxoplasmosis, Animal
biology
MESH: Escherichia coli
MESH: Toxoplasma
Brain
3. Good health
Vaccination
Lipid A
Infectious Diseases
medicine.anatomical_structure
Vaccines, Subunit
Recombinant DNA
Cytokines
Molecular Medicine
Antibody
Toxoplasma
Adjuvant
MESH: Vaccines, Synthetic
030231 tropical medicine
Antigens, Protozoan
Spleen
Microbiology
03 medical and health sciences
MESH: Brain
Adjuvants, Immunologic
Antigen
parasitic diseases
Escherichia coli
medicine
Animals
Humans
MESH: Antibodies, Protozoan
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
MESH: Mice
030304 developmental biology
MESH: Humans
General Veterinary
General Immunology and Microbiology
MESH: Protozoan Vaccines
Public Health, Environmental and Occupational Health
Toxoplasma gondii
Th1 Cells
biology.organism_classification
Disease Models, Animal
Toxoplasmosis, Animal
MESH: Th1 Cells
Immunology
Mice, Inbred CBA
biology.protein
MESH: Disease Models, Animal
MESH: Antigens, Protozoan
Subjects
Details
- Language :
- English
- ISSN :
- 0264410X
- Database :
- OpenAIRE
- Journal :
- Vaccine, Vaccine, Elsevier, 2007, 25 (21), pp.4301-11. ⟨10.1016/j.vaccine.2007.02.057⟩
- Accession number :
- edsair.doi.dedup.....d9d1d35e5a6c867b1e1096ec7031d6d1