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Portrayal of Punica granatum L. peel extract through High Performance Liquid Chromatography and antimicrobial activity evaluation
- Source :
- Brazilian Journal of Biology v.83 2023, Brazilian Journal of Biology, Instituto Internacional de Ecologia (IIE), instacron:IIE, Brazilian Journal of Biology, Vol 83 (2021)
- Publication Year :
- 2023
- Publisher :
- Instituto Internacional de Ecologia, 2023.
-
Abstract
- Increasing trend in antimicrobial resistance and failure of chemically synthesized antibiotics lead to discover alternative methods for the treatment of bacterial infections. Various medicinal plants are in use traditionally and their active compounds can be further applied for treatment of bacterial diseases. This study was designed to determine the antibacterial activity of Punica granatum (P. granatum L.) (pomegranate) peel extract against Enterobacteriaceae [Escherichia coli (E. coli), Salmonella Typhimurium (S. Typhimurium) and Shigella Dysenteriae (S. Dysenteriae)] and gram-positive bacterium [Staphylococcus aureus (Staph aureus)]. Methanolic extract of P. granatum L. peel was prepared by Soxhlet apparatus method. Total flavonoid and phenolic contents from the extract were determined by High Performance Liquid Chromatography (HPLC). The antibacterial activity of P. granatum L. peel extract was evaluated through agar well diffusion method. HPLC showed the range of phenolics (gallic acid, caffeic acid, benzoic acid, cinnamic acid) and flavonoid compounds. The chemical structures of flavonoid and phenolics found in the methanolic extract of P. granatum L. peel have been reported for the first time. The methanolic peel extract (50 ul) of yellow P. granatum L. showed 26, 10, 10 and 9mm zones of inhibition (ZOI) against S. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. The methanolic extract of red P. granatum L. (100 ul) showed 27, 8, 12 and 15 mm ZOI against Staph. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. Highest ZOI was observed against Staph. aureus. Many of the bacteria studied in the present work may cause serious gastrointestinal infections, which can lead to hemorrhagic diarrhea in children. These infections can be life-threatening to young children and the elderly. There is an incentive to find alternative control measures, such as plant and herbal extracts, especially in lesser-developed countries where traditional antibiotics may not be readily available.
- Subjects :
- Staphylococcus aureus
Shigella dysenteriae
QH301-705.5
Science
Flavonoid
medicine.disease_cause
Pomegranate
chemistry.chemical_compound
Anti-Infective Agents
Enterobacteriaceae
Caffeic acid
medicine
Escherichia coli
Humans
Gallic acid
Biology (General)
Child
Chromatography, High Pressure Liquid
Aged
P. granatum L
chemistry.chemical_classification
antimicrobial activity
biology
Traditional medicine
Plant Extracts
Botany
food and beverages
Antimicrobial
biology.organism_classification
QL1-991
chemistry
QK1-989
Punica
Child, Preschool
HPLC
General Agricultural and Biological Sciences
Antibacterial activity
Zoology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Brazilian Journal of Biology v.83 2023, Brazilian Journal of Biology, Instituto Internacional de Ecologia (IIE), instacron:IIE, Brazilian Journal of Biology, Vol 83 (2021)
- Accession number :
- edsair.doi.dedup.....d9f593bac524557716f3aeafe68b7552