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A new micropatterning method of soft substrates reveals that different tumorigenic signals can promote or reduce cell contraction levels
- Source :
- Lab on a Chip, Lab on a Chip, Royal Society of Chemistry, 2011, 11 (13), pp.2231-40. ⟨10.1039/c0lc00641f⟩, Lab on a Chip, 2011, 11 (13), pp.2231-40. ⟨10.1039/c0lc00641f⟩
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- International audience; In tissues, cell microenvironment geometry and mechanics strongly impact on cell physiology. Surface micropatterning allows the control of geometry while deformable substrates of tunable stiffness are well suited for the control of the mechanics. We developed a new method to micropattern extracellular matrix proteins on poly-acrylamide gels in order to simultaneously control cell geometry and mechanics. Microenvironment geometry and mechanics impinge on cell functions by regulating the development of intra-cellular forces. We measured these forces in micropatterned cells. Micropattern geometry was streamlined to orient forces and place cells in comparable conditions. Thereby force measurement method could be simplified and applied to large-scale experiment on chip. We applied this method to mammary epithelial cells with traction force measurements in various conditions to mimic tumoral transformation. We found that, contrary to the current view, all transformation phenotypes were not always associated to an increased level of cell contractility.
- Subjects :
- Cell physiology
Materials science
Biomedical Engineering
Acrylic Resins
Bioengineering
02 engineering and technology
cell geometry
surface micropatterning
Biochemistry
Extracellular matrix
extracellular matrix proteins
03 medical and health sciences
mammary epithelial cells
cell mechanics
Cell contraction
Cell Line, Tumor
medicine
Humans
human
measurement method
[SDV.BDD]Life Sciences [q-bio]/Development Biology
030304 developmental biology
Cell Proliferation
Mechanical Phenomena
0303 health sciences
Measurement method
Tractive force
Stiffness
Reproducibility of Results
General Chemistry
021001 nanoscience & nanotechnology
Control cell
intracellular forces
Biomechanical Phenomena
Mammalia
cytoskeleton architecture
Microtechnology
medicine.symptom
0210 nano-technology
Gels
Biomedical engineering
Micropatterning
Subjects
Details
- Language :
- English
- ISSN :
- 14730197 and 14730189
- Database :
- OpenAIRE
- Journal :
- Lab on a Chip, Lab on a Chip, Royal Society of Chemistry, 2011, 11 (13), pp.2231-40. ⟨10.1039/c0lc00641f⟩, Lab on a Chip, 2011, 11 (13), pp.2231-40. ⟨10.1039/c0lc00641f⟩
- Accession number :
- edsair.doi.dedup.....da0823257c0e7945f1cd734a75411903