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Isolation and characterization of antifungal peptides produced by Bacillus amyloliquefaciens LBM5006
- Source :
- The Journal of Microbiology. 48:791-797
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- Bacillus amyloliquefaciens LBM 5006 produces antagonistic activity against pathogenic bacteria and phytopathogenic fungi, including Aspergillus spp., Fusarium spp., and Bipolaris sorokiniana. PCR analysis revealed the presence of ituD, but not sfp genes, coding for iturin and surfactin, respectively. The antimicrobial substance produced by this strain was isolated by ammonium sulfate precipitation, gel filtration chromatography and 1-butanol extraction. The ultraviolet spectrum was typical of a polypeptide and the infrared spectrum indicates the presence of peptide bonds and acyl group(s). The antimicrobial substance was resistant to proteolytic enzymes and heat treatment, and was reactive with ninhydrin. Mass spectroscopy analysis indicated that B. amyloliquefaciens LBM 5006 produces two antimicrobial peptides, with main peaks at m/z 1,058 Da and 1,464 Da, corresponding to iturin-like and fengycin-like peptides, respectively. B. amyloliquefaciens LBM 5006 showed significant activity against phytopatogenic fungi, showing potential for use as a biocontrol agent or production of antifungal preparations.
- Subjects :
- Antifungal Agents
Bacillus amyloliquefaciens
Molecular Sequence Data
Antimicrobial peptides
Bacillus
Microbial Sensitivity Tests
Chemical Fractionation
Applied Microbiology and Biotechnology
Microbiology
Mass Spectrometry
chemistry.chemical_compound
Bacteriocin
Ammonium sulfate precipitation
biology
Spectrum Analysis
Fungi
Proteolytic enzymes
Sequence Analysis, DNA
General Medicine
Bipolaris
biology.organism_classification
Molecular Weight
chemistry
Biochemistry
Ninhydrin
Chromatography, Gel
Peptides
Surfactin
Subjects
Details
- ISSN :
- 19763794 and 12258873
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- The Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....da11f7ca89f936b33f4c10c1bcf8aadb