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Highly sensitive enclosed multilayer paper-based microfluidic sensor for quantifying proline in plants
- Source :
- Analytica Chimica Acta. 1105:169-177
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Free proline, termed proline, is a biomarker used for diagnosing drought stress in plants. A previously developed proline–ninhydrin reaction-based paper sensor could quickly and easily detect proline, but it was limited by low sensitivity. In this study, we developed an enclosed multilayer paper-based microfluidic sensor with high sensitivity for the quantitative detection of proline in plants. The multilayer paper-based sensor was manufactured using simple wax printing and origami methods, and contained an internal mixing channel to allow good mixing of the proline with ninhydrin, increasing the proline–ninhydrin reactivity and providing accurate and sensitive proline detection. By preloading ninhydrin onto the sample loading area, uniform coloration of the sensing window was achieved, allowing quantitative analysis of various proline concentrations using a constant reaction time. Only the sensing window and sample loading area were exposed to limit sample evaporation and contamination from the external environment. The LOD of the fabricated sensor was 23 μM, which is approximately 29-fold lower than that of the previously proposed paper sensor (657 μM). Samples were extracted from A. thaliana plants subjected to drought stress for proline detection. The proline concentrations measured using the developed paper sensor and a spectrophotometric method were not statistically significant at a confidence level of 95%. Therefore, the developed sensor can be applied to measure proline concentrations precisely in the field with a low detection limit. The developed paper-based sensor can be used to detect the early stages of drought in plants and thus improve crop productivity.
- Subjects :
- Paper
Proline
Surface Properties
Microfluidics
Arabidopsis
Evaporation
02 engineering and technology
01 natural sciences
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
Environmental Chemistry
Particle Size
Spectroscopy
Detection limit
Wax
Molecular Structure
Chemistry
fungi
010401 analytical chemistry
Ninhydrin
food and beverages
Microfluidic Analytical Techniques
Contamination
021001 nanoscience & nanotechnology
0104 chemical sciences
visual_art
visual_art.visual_art_medium
Indicators and Reagents
0210 nano-technology
Biological system
Quantitative analysis (chemistry)
Subjects
Details
- ISSN :
- 00032670
- Volume :
- 1105
- Database :
- OpenAIRE
- Journal :
- Analytica Chimica Acta
- Accession number :
- edsair.doi.dedup.....da281ba63ac9c089b86572ba7fdf85c4