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Study on the expression of PAK4 and P54 protein in breast cancer
- Source :
- World Journal of Surgical Oncology
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Background Previous evidence have demonstrated that p21-activated kinase PAK4 was correlated with breast cancer. The aim of this paper is to study the expression and interaction of p21-activated kinase (pAK)-4 and P54 protein in breast cancer. Methods A total of 80 patients were enrolled in our study (breast fibroma n = 20, breast noninvasive cancer n = 20, early breast invasive cancer n = 20, and advanced breast invasive cancer). The expression of PAK4 was detected by immunohistochemical S-P method, and the relationship between them and the different pathological characteristics were compared. The subcellular localization of P54 and PAK4 in vitro was observed by immunofluorescence assay. Results The expression of both PAK4 and P54 in breast cancer was much higher than that in breast fibroma. Meanwhile, we found that both PAK4 and P54 increased gradually as breast cancer progressed (advanced invasive > early invasive > noninvasive). The positive staining of P54 were mainly located in the cytoplasm, especially around the nucleus. There was no significant stained region in the cell matrix. The P54 localization in the cytoplasm was verified by confocal experiment, and the PAK4 was co-localized. Conclusions PAK4 and P54 proteins may be used as molecular markers for diagnosis and treatment of breast cancer.
- Subjects :
- Adult
0301 basic medicine
CA15-3
Fluorescent Antibody Technique
CA 15-3
Breast Neoplasms
Fibroma
Immunofluorescence
Chloroplast Proteins
03 medical and health sciences
Breast cancer
0302 clinical medicine
Surgical oncology
Endoribonucleases
Biomarkers, Tumor
medicine
Humans
Neoplasm Invasiveness
skin and connective tissue diseases
Aged
Neoplasm Staging
P54
medicine.diagnostic_test
business.industry
Research
Cancer
Middle Aged
Prognosis
medicine.disease
030104 developmental biology
p21-Activated Kinases
Oncology
PAK4
030220 oncology & carcinogenesis
Cancer research
Immunohistochemistry
Female
Surgery
business
Follow-Up Studies
Subjects
Details
- ISSN :
- 14777819
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- World Journal of Surgical Oncology
- Accession number :
- edsair.doi.dedup.....da43f6d15f0b800c8569974f998b6009