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Fatty Acid Transporter CD36 Mediates Hypothalamic Effect of Fatty Acids on Food Intake in Rats
- Source :
- PLoS ONE, Vol 8, Iss 9, p e74021 (2013), PLoS ONE, PLoS ONE, Public Library of Science, 2013, 8 (9), pp.e74021. ⟨10.1371/journal.pone.0074021⟩, PLoS ONE, 2013, 8 (9), pp.e74021. ⟨10.1371/journal.pone.0074021⟩, Plos One 9 (8), e74021. (2013), PLoS ONE, Public Library of Science, 2013, 8 (9), pp.e74021. 〈10.1371/journal.pone.0074021〉
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Subject Areas: carotid arteries; emulsions; fatty acids; gene expression; heparin; hypothalamus; neurons; oxidation.; International audience; Variations in plasma fatty acid (FA) concentrations are detected by FA sensing neurons in specific brain areas such as the hypothalamus. These neurons play a physiological role in the control of food intake and the regulation of hepatic glucose production. Le Foll et al. previously showed in vitro that at least 50% of the FA sensing in ventromedial hypothalamic (VMH) neurons is attributable to the interaction of long chain FA with FA translocase/CD36 (CD36). The present work assessed whether in vivo effects of hypothalamic FA sensing might be partly mediated by CD36 or intracellular events such as acylCoA synthesis or β-oxidation. To that end, a catheter was implanted in the carotid artery toward the brain in male Wistar rats. After 1 wk recovery, animals were food-deprived for 5 h, then 10 min infusions of triglyceride emulsion, Intralipid +/- heparin (IL, ILH, respectively) or saline/heparin (SH) were carried out and food intake was assessed over the next 5 h. Experimental groups included: 1) Rats previously injected in ventromedian nucleus (VMN) with shRNA against CD36 or scrambled RNA; 2) Etomoxir (CPT1 inhibitor) or saline co-infused with ILH/SH; and 3) Triacsin C (acylCoA synthase inhibitor) or saline co-infused with ILH/SH. ILH significantly lowered food intake during refeeding compared to SH (p
- Subjects :
- CD36 Antigens
Male
Microdialysis
medicine.medical_specialty
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
CD36
Hypothalamus
Gene Expression
lcsh:Medicine
Models, Biological
Eating
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
In vivo
Internal medicine
medicine
Animals
lcsh:Science
Phospholipids
030304 developmental biology
2. Zero hunger
chemistry.chemical_classification
0303 health sciences
Multidisciplinary
Triglyceride
biology
Fatty Acids
lcsh:R
Neurosciences
[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Fatty acid
Feeding Behavior
Fatty Acid Transport Proteins
Rats
Soybean Oil
Triacsin C
Endocrinology
chemistry
[ SDV.NEU.NB ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Neurons and Cognition
biology.protein
Emulsions
lcsh:Q
Proto-Oncogene Proteins c-fos
030217 neurology & neurosurgery
Etomoxir
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....da473c1c930c99a2c6b2846fc160da59