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Effects of compound K, an enteric microbiome metabolite of ginseng, in the treatment of inflammation associated colon cancer
- Source :
- Oncology Letters
- Publication Year :
- 2018
- Publisher :
- Spandidos Publications, 2018.
-
Abstract
- Ginsenoside Rb1, a major component of different ginseng species, can be bioconverted into compound K by gut microbiota, and the latter possess much stronger cancer chemopreventive potential. However, while the initiation and progression of colorectal cancer is closely associated with gut inflammation, to date, the effects of compound K on inflammation-linked cancer chemoprevention have not been reported. In the present study, liquid chromatography quadrupole time-of-flight mass spectrometry analysis was applied to evaluate the biotransformation of Rb1 in American ginseng by human enteric microflora. The in vitro inhibitory effects of Rb1 and compound K were compared using the HCT-116 and HT-19 human colorectal cancer cell lines by a MTS assay. Cell cycle and cell apoptosis were assayed using flow cytometry. Using ELISA, the anti-inflammatory effects of Rb1 and compound K were compared for their inhibition of interleukin-8 secretion in HT-29 cells, induced by lipopolysaccharide. The results revealed that compound K is the major intestinal microbiome metabolite of Rb1. When compared with Rb1, compound K had significantly stronger anti-proliferative effects in HCT-116 and HT-29 cell lines (P
- Subjects :
- 0301 basic medicine
Cancer Research
liquid chromatography quadrupole time-of-flight mass spectrometry
Metabolite
Cell
colorectal cancer
HT-29 cancer cells
Pharmacology
Gut flora
HCT-116 cancer cell
Flow cytometry
anti-proliferation
03 medical and health sciences
chemistry.chemical_compound
Ginseng
0302 clinical medicine
ginsenoside Rb1
medicine
medicine.diagnostic_test
biology
apoptosis
Cancer
compound K
Articles
Cell cycle
medicine.disease
biology.organism_classification
anti-inflammation
eye diseases
3. Good health
030104 developmental biology
medicine.anatomical_structure
Oncology
chemistry
Apoptosis
030220 oncology & carcinogenesis
cell cycle
Subjects
Details
- ISSN :
- 17921082 and 17921074
- Database :
- OpenAIRE
- Journal :
- Oncology Letters
- Accession number :
- edsair.doi.dedup.....da58b12290363495f6f843eeef2c3dda