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Antioxidant enzymatic defence systems in sheep corpus luteum throughout pregnancy

Authors :
Kaïs H, Al-Gubory
Philippe, Bolifraud
Guy, Germain
Annie, Nicole
Irène, Ceballos-Picot
Irène, Ceballos-Bicot
Source :
Reproduction. 128:767-774
Publication Year :
2004
Publisher :
Bioscientifica, 2004.

Abstract

The activities of copper, zinc-superoxide dismutase (SOD1), manganese SOD (SOD2), glutathione peroxidase (GPX), glutathione reductase (GSSG-R) and glutathioneS-transferase (GST) were studied in sheep corpora lutea (CL) obtained on days 15, 40, 60, 80 and 128 of pregnancy. Maintained enzymatic activity of SOD1, SOD2, GPX, GSSG-R and GST were found in the sheep CL throughout pregnancy. Enzymatic activity of SOD1, GPX and GST increased significantly from day 15 to day 40 of pregnancy, and thereafter remained constant until day 128. SOD2 and GSSG-R activities were not different between any days of pregnancy examined. Apoptotic luteal cells identified by the terminal deoxynucleotidyl transferase-mediated fluorescein-dUTP nick-end labelling were very rarely observed, and their incidence (less than 0.5%) was not different between days of pregnancy. These results showed that the activities of antioxidant enzymes in the sheep CL are subject to major changes during early pregnancy, suggesting that the CL of early pregnancy may be rescued from luteolysis through increasing activities of key antioxidant enzymes and inhibition of apoptosis. Maintained levels of antioxidant enzymes in the CL throughout pregnancy may be linked to reactive oxygen species continuously generated in the steroidogenically active luteal cells, and may be involved in the maintenance of luteal steroidogenic activity and cellular integrity.

Details

ISSN :
17417899 and 14701626
Volume :
128
Database :
OpenAIRE
Journal :
Reproduction
Accession number :
edsair.doi.dedup.....da629a8156e87a6dffe5cfbe46af70ca
Full Text :
https://doi.org/10.1530/rep.1.00389