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Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC‑1
- Source :
- Molecular medicine reports. 8(6)
- Publication Year :
- 2013
-
Abstract
- The aim of the present study was to investigate the effect of realgar on the viability, proliferation and apoptosis in the human chronic lymphocytic leukemia (CLL) cell line, MEC‑1. Potential mechanisms mediating the effect were also explored in the experiment. Cultured MEC‑1 cells were incubated with various concentrations of realgar for 24, 48 and 72 h. A WST‑8 assay was employed to evaluate the effect on cell viability. Inhibitory effects on cell proliferation were determined using a 5‑bromodeoxyuridine cell proliferation ELISA. The apoptotic effect on MEC‑1 cells was evaluated by annexin V‑fluorescein isothiocyanate/propidium iodide dual staining, followed by flow cytometry. Quantitative polymerase chain reaction was performed to determine the mRNA expression levels of BCL2‑associated X protein (BAX), BCL2‑like 1 (Bcl-xL), v‑myc myelocytomatosis viral oncogene homolog (avian; c‑Myc) and cyclin‑dependent kinase inhibitor 1A (p21). It was found that viability and proliferation were significantly reduced while apoptotic rates increased in MEC‑1 cells following exposure to realgar. Furthermore, mRNA expression of BAX and c‑Myc was upregulated and downregulated, respectively, in realgar‑treated MEC‑1 cells. In conclusion, the results showed that realgar inhibits viability and prolife-ration and induces apoptosis of MEC‑1 cells in a dose‑ and time‑dependent manner. The effect may depend on the mitochondrial apoptosis pathway. The results of the present study may be beneficial in the identification of a new target therapy for CLL.
- Subjects :
- Cyclin-Dependent Kinase Inhibitor p21
Cancer Research
Transcription, Genetic
Cell Survival
bcl-X Protein
Down-Regulation
Apoptosis
Biology
Realgar
Sulfides
Biochemistry
Arsenicals
Flow cytometry
Proto-Oncogene Proteins c-myc
chemistry.chemical_compound
Cell Line, Tumor
Genetics
medicine
Humans
Viability assay
Propidium iodide
RNA, Messenger
Molecular Biology
Cell Proliferation
bcl-2-Associated X Protein
medicine.diagnostic_test
Cell growth
Gene Expression Regulation, Leukemic
Cell cycle
Molecular biology
Leukemia, Lymphocytic, Chronic, B-Cell
Cell biology
Oncology
chemistry
Cell culture
Molecular Medicine
Drug Screening Assays, Antitumor
Subjects
Details
- ISSN :
- 17913004
- Volume :
- 8
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecular medicine reports
- Accession number :
- edsair.doi.dedup.....da7553f7a7e2ac452b42d54b1efadbf3